Growth and Cyanide Degradation of Azotobacter vinelandii in Cyanide-Containing Wastewater System = Growth and Cyanide Degradation of Azotobacter vinelandii in Cyanide-Containing Wastewater System

다국어 초록 (Multilingual Abstract)

Azotobacter vinelandii, a strict aerobic nitrogen-fixing bacterium, has been extensively studied with regard to the ability of N2-fixation due to its high expression of nitrogenase and fast growth. Because nitrogenase can also reduce cyanide to ammonia and methane, cyanide degradation by A. vinelandii has been studied for the application in the bioremediation of cyanide-contaminated wastewater. Cyanide degradation by A. vinelandii in NFS (nitrogen-free sucrose) medium was examined in terms of cell growth and cyanide reduction, and the results were applied for cyanide-contaminated cassava mill wastewater. From the NFS medium study in the 300 ml flask, it was found that A. vinelandii in the early stationary growth phase could reduce cyanide more rapidly than the cells in the exponential growth phase, and 84.4% of cyanide was degraded in 66 h incubation upon addition of 3.0 mM of NaCN. The resting cells of A. vinelandii could also reduce cyanide concentration by 90.4% with 3.0 mM of NaCN in the large-scale (3 L) fermentation with the same incubation time. Finally, the optimized conditions were applied to the cassava mill wastewater bioremediation, and A. vinelandii was able to reduce the cyanide concentration by 69.7% after 66 h in the cassava mill wastewater containing 4.0 mM of NaCN in the 3 L fermenter. Related to cyanide degradation in the cassava mill wastewater, nitrogenase was the responsible enzyme, which was confirmed by methane production. These findings would be helpful to design a practical bioremediation system for the treatment of cyanide-contaminated wastewater.

참고문헌 (Reference)

1 Baxter, J., "The current and future applications of microorganism in the bioremediation of cyanide contamination" 90 : 1-7, 2006

2 Kim, M., "Synthesis, structure and physical properties of trinuclear M3tdt3(PEt3)3 (M=FeII, CoII) clusters containing metal-metal bonds" 26 : 4557-4566, 2007

3 Jaehong Han, "Structure and Physical Properties of Copper Thiomolybdate Complex, (nBu4N)3[MoS4Cu3Cl4]" 대한화학회 29 (29): 2299-2302, 2008

4 APHA, AWWA, "Standard Methods for the Examination of Water and Wastewater" American Public Health Association 1995

5 Newton, W. E., "Nitrogenases: Distribution, composition, structure and function, In New Horizons in Nitrogen Fixation" Kluwer Academic 5-18, 1993

6 Li, J., "Nitrogenase reactivity: Cyanide as substrate and inhibitor" 21 : 4393-4402, 1982

7 Gapes, D. J., "Nitrogen fixation in treatment of pulp and paper wastewaters" 40 : 85-92, 1999

8 Burgess, B. K., "Mechanism of molybdenum nitrogenase" 96 : 2983-3011, 1996

9 Stranberg, G. W., "Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii" 14 : 25-31, 1968

10 Drochioiu, G., "Fast and highly selective determination of cyanide with 2,2-dihydroxy-1,3-indanedione" 56 : 1163-1165, 2002

11 Adjei, M. D., "Factors affecting the biodegradation of cyanide by Burkholderia cepacia strain C-3" 89 : 274-277, 2000

12 Han, J., "Differentiation of acetylenereduction sites by stereoselective proton addition during Azotobacter vinelandii nitrogenase-catalyzed C2D2 reduction" 43 : 2947-2956, 2004

13 Kaewkannetra, K., "Cyanide removal from cassava mill wastewater using Azotobactor vinelandii TISTR 1094 with mixed microorganisms in activated sludge treatment system" 172 : 224-228, 2009

14 Watanabe, A., "Cyanide hydrolysis in a cyanide-degrading bacterium, Pseudomonas stutzeri AK61, by cyanidase" 144 : 1677-1682, 1998

15 Eisler, R., "Cyanide hazards to fish, wildlife and invertebrates" U.S. Department of the Interior, Fish and Wildlife Service 1991

16 White, J. M., "Conversion of cyanide to formate and ammonia by Pseudomonas from industrial wastewater" 3 : 263-272, 1988

17 Kao, C. M., "Biotransformation of cyanide to methane and ammnonia by Klebsiella oxytoca" 50 : 1055-1061, 2003

18 Ehaliotis, C., "Adaptation and population dynamics of Azotobacter vinelandii during aerobic biological treatment of olive-mill wastewater" 30 : 301-311, 1999