RISS 검색 - 국내학술지논문 상세보기

다국어 초록 (Multilingual Abstract)

Background/Aims High-resolution extracellular mapping has improved our understanding of bioelectric slow-wave and spike-burst activity in the small intestine. The spatiotemporal correlation of electrophysiology and motility patterns is of critical interest to intestinal function but remains incompletely defined.
Methods Intestinal jejunum segments from in vivo pigs and rabbits were exteriorized, and simultaneous high-resolution extracellular recordings and video recordings were performed. Contractions were quantified with strain fields, and the frequencies and velocities of motility patterns were calculated. The amplitudes, frequencies, and velocities of slow-wave propagation patterns and spike-bursts were quantified and visualized. In addition, the duration, size and energy of spike-burst patches were quantified.
Results Slow-wave associated spike-bursts activated periodically at 10.8 ± 4.0 cycles per minute (cpm) in pigs and 10.2 ± 3.2 cpm in rabbits, while independent spike-bursts activated at a frequency of 3.2 ± 1.8 cpm. Independent spike-bursts had higher amplitude and longer duration than slow-wave associated spike-bursts (1.4 ± 0.8 mV vs 0.1 ± 0.1 mV, P < 0.001; 1.8 ± 1.4 seconds vs 0.8 ± 0.3 seconds, P < 0.001 in pigs). Spike-bursts that activated as longitudinal or circumferential patches were associated with contractions in the respective directions. Spontaneous peristaltic contractions were elicited by independent spike-bursts and travelled slower than slow-wave velocity (3.7 ± 0.5 mm/sec vs 10.1 ± 4.7 mm/sec, P = 0.007). Cyclic peristaltic contractions were driven by slow-wave associated spike-bursts and were coupled to slow-wave velocity and frequency in rabbit (14.2 ± 2.3 mm/sec vs 11.5 ± 4.6 mm/sec, P = 0.162; 11.0 ± 0.6 cpm vs 10.8 ± 0.6 cpm, P = 0.970).
Conclusions Motility patterns were dictated by patterns of spike-burst patches. When spike-bursts were coupled to slow-waves, periodic motility patterns were observed, while when spike-bursts were not coupled to slow-waves, spontaneous aperiodic motility patterns were captured.

참고문헌 (Reference)

1 Grasa L, "The role of Ca2+ in the contractility of rabbit small intestine in vitro" 55 : 639-650, 2004

2 Øigaard A, "The relative significance of electrical spike potentials and intraluminal pressure waves as quantitative indicators of motility" 19 : 797-803, 1974

3 Huizinga JD, "The origin of segmentation motor activity in the intestine" 5 : 3326-, 2014

4 Cannon WB, "The movements of the intestines studied by means of the Röntgen rays" 7 : 72-75, 1902

5 Yassi R, "The gastrointestinal electrical mapping suite(GEMS) : software for analyzing and visualizing high-resolution(multi-electrode)recordings in spatiotemporal detail" 12 : 60-, 2012

6 Angeli TR, "The bioelectrical basis and validity of gastrointestinal extracellular slow wave recordings" 591 : 4567-4579, 2013

7 Seerden TC, "Spatiotemporal electrical and motility mapping of distension-induced propagating oscillations in the murine small intestine" 289 : G1043-G1051, 2005

8 Lammers WJEP, "Spatial and temporal coupling between slow waves and pendular contractions" 289 : G898-G903, 2005

9 Ozaki H, "Simultaneous measurement of membrane potential, cytosolic Ca2+, and tension in intact smooth muscles" 260 (260): C917-C925, 1991

10 Lammers WJEP, "Similarities and differences in the propagation of slow waves and peristaltic waves" 283 : G778-G786, 2002