척수 손상 토끼의 근 신장 반사에서 척수강내 바클로펜 주입법의 효과 = The Effect of Intrathecal Baclofen Injection on Muscle Stretch Reflex in Spinal Cord Injured Rabbit

다국어 초록 (Multilingual Abstract)

Objective: To develop an experimental model that is useful to evaluate the effect of antispastic medication administered intrathecally and to titrate the intrathecal baclofen effect quantitatively by using muscle stretch reflex.
Method: Ten rabbits were laminectomized posteriorly and intrathecal catheter was inserted. Then the spinal cords were contused by 12.5 g×20 cm weight drop around 12th thoracic vertebra. After 8～12 days, muscle stretch reflex was measured before intrathecal baclofen injection (ITBI) and after ITBI 30 minutes, 60 minutes, and 120 minutes. Rabbits' triceps surae were dissected and stretched 5 mm at the rate of 2 mm/sec using a step motor. The change of muscle tension was graphed into the time (length)-tension curve. The slope in the time (length)-tension curve was defined as stiffness index (SI).
Results: The measurement of muscle stretch reflex was available in 5 of 10 spinal cord injured rabbits. The proportion of SI reduced significantly at 60 minutes and 120 minutes compared to baseline (p=0.005, p＜0.001).
Conclusion: Our data proved the antispastic effect of ITBI quantitatively by using muscle stretch reflex. We concluded that the quantitative measurement method of the antispastic effect of ITBI will be useful in evaluating antispastic effect by intrathecal administration of the other antispastic medications.

참고문헌 (Reference)

1 "페놀 차단술의 말초 신경 차단 효과의 적정화:전기생리학적 및 역학적 측정" 23 : 1076-1082, 1999

2 "척수강 내 바클로펜 주입의 효과" 22 : 1239-1248, 1998

3 "척수 손상 토끼의 신장 반사의 변화:경직의 동물 모델" 26 : 37-45, 2002

4 "척수 손상 토끼의 근 신장 반사에서 Gabapentin의 효과" 27 : 701-707, 2003

5 "mechanisms of action" 107-116,

6 "The distribution of medication along the spinal canal after chronic intrathecal administration" 33 : 226-230, 1993

7 "Spasticity and muscle contracture following stroke" 119 : 1737-1749, 1996

8 "Reduced spinal reflexes following intrathecal baclofen in the rabbit" 54 : 191-194, 1984

9 "Prospective study on the use of bolus intrathecal baclofen for spastic hypertonia due to acquired brain injury" 77 : 461-466, 1996

10 "New assessment technique for evaluation of posttraumatic spinal cord function in the rat" 13 : 741-754, 1996

11 "Neurosurgical treatment of spasticity:selective posterior rhizotomy and intrathecal baclofen" 58 : 3-13, 1992

12 "Management of spasticity" WB Saunders Company 585-592, 1996

13 "Lumbar catheterization of the spinal subarachnoid space in the rat" 65 : 167-172, 1996

14 "Intrathecal baclofen therapy for spastic hypertonia" 12 : 923-938, 2001

15 "Intrathecal baclofen for severe spinal spasticity" 320 : 1517-1521, 1989

16 "Intrathecal baclofen for severe spasticity secondary to spinal cord injury" 27 : 767-774, 1993

17 "Drugs used to treat spasticity" 59 : 487-495, 2000

18 "Direct spinal effect of intrathecal and exrtradural midazolam on visceral noxious stimulation in rabbits" 70 : 642-646, 1993

19 "Comparison of the antinociceptive and antispastic action of baclofen after systemic and intrathecal administration in intact,acute,ad chronic spinal rats" 684 : 8-18, 1995

20 "Animal models of spinal cord contusion injuries" 49 : 161-172, 1999

21 "A rabbit model for evaluation of spinal anesthesia:chronic cannulation of the subarachnoid space" 71 : 529-535, 1990