Study on Alternative Testing Methods Using Zebrafish Embryos

다국어 초록 (Multilingual Abstract)

With the advancement of technology, alternative methods to animal testing are continuously being developed, prompting a reassessment of the necessity of animal experiments. Following the 3R principles(Replacement, Reduction, and Refinement), efforts are being made to minimize the use of experimental animals and reduce their suffering.
In this study, an acute toxicity test was conducted using zebrafish embryos with m-phenylenediamine, a test substance with predicted toxicity. The purpose of this study is to evaluate the potential of these alternative testing methods to replace conventional animal testing by verifying whether the predicted toxicity of the test substance aligns with the actual results.
In the acute toxicity test using zebrafish embryos, the LC50(48h) was determined to be 36.862 mg/L, and the LC50(96h) was 27.122 mg/L. These results suggest that the zebrafish embryo model provides an ethical and efficient approach for chemical substance evaluation.
This study is expected to contribute to addressing ethical issues related to animal testing and ensuring scientific validity by demonstrating the feasibility of these alternative testing methods.