Selective Elimination of Clonogenic Leukemic Cells by Merocyanine-540 Mediated Photosensitization for Autologous Bone Marrow Transplantation

다국어 초록 (Multilingual Abstract)

In order to achieve a successful autologous bone marrow transplantation, we should overcome the residual leukemic clonogenic cells which is possible contamination of the harvest- ed bone marrow. The experiments reported here were designed to test the in vitro dose dependent photodynamic effect of merocyanine-540 (MC-540) on K563, HL-60 and chronic myelogenous leukemic cells.
1. MC-540 without light exposure was not remarkably cytotoxic, a concentration of 20 ㎍/ml or less MC-540 was adequate.
2. To preserve enough normal hematopoietic cells, a concentrations of 20 ㎍/ml or less MC-540 with light exposure was adequate.
3. The most optimal condition of photosensitization to eliminate clonogenic leukemic cells selectively was 20 ㎍/ml MC-540 for 60 minutes of light exposure.
4. Renewal capacity of clonogenic leukemic cells was lost even though they survived after photo-purging.
5. Normal marrow cells contaminated with K562 and HL-60 cells stimulated to complete remission marrow with minimal residual leukemic cells were effectively purged.
6. The purging effect on leukemic cells of MC-540 was added to that of 4-hydroperoxycyclosphosphamide and heat.
MC-540 with photosensitization seems to be useful for ex-viyo purging of residual leukemic cells in autologous bone marrow transplantation.