지리산오갈피, 가시오갈피, 오갈피나무, 오가나무 추출물의 항염증, 피부장벽개선, 항노화 효능 비교 = Comparison of Anti-inflammatory, Skin Barrier Improvement, and Anti-aging Efficacy of Eleutherococcus divaricatus var. chiisanensis and various Eleutherococcus Genus Extract

다국어 초록 (Multilingual Abstract)

Inflammation caused by active oxygen and the resulting barrier damage have been consistently pointed out as the cause of wrinkle formation. In this study, effective index ingredient search and efficacy analysis were performed to verify the value of use as a functional cosmetic material related to antioxidant, anti-inflammatory and skin barrier improvement, and anti-aging for extracts of four types of Eleutherococcus divaricatus var. chiisanensis (ED), Eleutherococcus senticosus (EN), Eleutherococcus sessiliflorus (ES), and Eleutherococcus sieboldianus (EI) belonging to the Eleutherococcus genus. To identify the effective index composition, the content of the ingredients was measured by high-performance liquid chromatography. The content of eleutheroside E and chlorogenic acid was the highest in ED among the Eleutherococcus genus. As for anti-oxidant activity, DPPH radical scavenging activity was the highest in ED.
In anti-inflammatory effects, ED extracts inhibited nitric oxide generation in inflammatory macrophage cells due to lipopolysaccharide by 40% at 100 μg/mL. In the case of IL-6 inhibition, which is known as a pro-inflammatory cytokine, ED showed 41% inhibition at 100 μg/mL. In addition, filaggrin and involucrin, which are skin barrier-related factors, were increased by 2.5 times and 1.6 times, respectively, in 100 μg/mL of ED extracts, and as for the collagenase, which is a wrinkle-related factor, ED extract showed 29% efficacy at 100 μg/mL. Thus, these result suggested that ED extract, among the four Eleutherococcus genus, can be used as a cosmetic ingredient for suppressing inflammation in the skin, reinforcing the skin barrier, and reducing wrinkles.

국문 초록 (Abstract)

활성산소로 인한 염증 유발과 그로 인한 장벽손상은 꾸준히 주름생성의 원인으로 지목되어 왔다. 본연구에서는 오갈피나무속에 속해있는 지리산오갈피와 가시오갈피, 오갈피나무, 오가나무 4종의 추출물에 대한항산화, 항염증 및 피부 장벽개선, 항노화 관련 기능성 화장품 소재로서의 활용가치를 검증하고자 유효 지표성분탐색과 효능 분석을 수행하였다. 먼저, 오갈피나무속 식물인 지리산오갈피와 가시오갈피, 오갈피나무, 오가나무각각을 50% 에탄올을 용매로 추출, 농축, 동결건조 하여 시료를 제조하였다. 유효 지표성분탐색을 위해 주요지표 성분인 eleutheroside E와 오갈피나무속에서 주로 검출되는 폴리페놀류 중 chlorogenic acid, caffeic acid 에 대하여 고성능 액체크로마토그래피를 통해 성분 함량을 측정하였으며, 오갈피나무속 중 지리산오갈피에서eleutheroside E와 chlorogenic acid의 함량이 가장 높은 것을 확인하였다. 항산화활성은 DPPH 라디칼 소거활성이 지리산오갈피에서 가장 높았으며, 항염증 효능의 경우 LPS로 염증이 유발된 대식세포 내 NO 생성 억제효능은 가시오갈피, 오가나무, 오갈피나무 추출물 100 μg/mL에서 각각 25%, 26%, 32% 일 때 지리산오갈피추출물 100 μg/mL는 40% 억제 효능을 보였고 pro-inflammatory cytokine으로 알려진 IL-6 발현 억제 효능은가시오갈피, 오가나무, 오갈피나무 추출물 100 μg/mL 농도에서 각각 20%, 36%, 23% 일 때 지리산오갈피추출물 100 μg/mL는 41% 억제 효능을 보였다. 또한, 지리산오갈피 추출물 100 μg/mL에서 피부장벽 관련 인자인filaggrin, involucrin의 발현이 각각 2.5 배, 1.6 배 증가되었으며, 주름관련 인자인 콜라겐분해효소는 29% 억제 효능을 나타내어 오갈피나무속 4 종 중 지리산오갈피는 피부의 염증 억제와 장벽강화, 주름개선용 화장품소재로써 활용가능하리라 판단된다.

참고문헌 (Reference)

1 이태범 ; 소양강 ; 김세율 ; 황지영, "화장품 소재로서의 꽃 10 종 에탄올추출물 생리활성 특성연구" 한국약용작물학회 28 (28): 260-275, 2020

2 곽충실 ; 양지원, "인간피부세포에서 복숭아꽃 추출물의 투여가 자외선 B 조사에 의한 활성산소와 Matrix Metalloproteinases 생성 유도를 억제하는 효과" 한국피부과학연구원 14 (14): 179-190, 2016

3 안혜정 ; 육창수 ; 김형춘 ; 고성권, "오가피속 식물 잎의 식물화학 성분 비교" 대한약학회 61 (61): 90-95, 2017

4 N. Tsuji, "The role of elastases secreted by fibroblasts in wrinkle formation : implication through selective inhibition of elastase activity" 74 (74): 283-, 2001

5 P. M. Steinert, "The proteins elafin, filaggrin, keratin intermediate filaments, loricrin, and small proline-rich proteins 1 and 2 are isodipeptide cross-linked components of the human epidermal cornified cell envelope" 270 (270): 17702-, 1995

6 Yu. S. Ovodov, "The glycosides of Eleutherococcus senticosusI I. The structure of eleutherosides A, B1, C, and D" 3 : 53-, 1967

7 Yu. S. Ovodov, "The glycosides of Eleutherococcus senticoccus max. I. isolation and some properties of eleutherosides B and E" 1 (1): 1-, 1965

8 C. Xie, "The açaí flavonoid velutin is a potent anti-inflammatory agent : blockade of LPSmediated TNF-α and IL-6 production through inhibiting NF-κB activation and MAPK pathway" 23 (23): 1184-, 2012

9 Y. S. Rim, "Screening of antioxidants and antimicrobial activity in native plants" 8 (8): 342-, 2000

10 S. Natesan, "Peroxisome proliferator-activated receptor-α agonist and all-trans retinoic acid induce epithelial differentiation of subcutaneous adipose-derived stem cells from debrided burn skin" 120 (120): 9213-, 2019

11 A. J. Choi, "History of Korean Horticulture" Korean Society for Horticultural Science 420-, 2013

12 Min-Ja Park ; Young-Seuk Bae, "Fermented Acanthopanax koreanum Root Extract Reduces UVB- and H2O2-Induced Senescence in Human Skin Fibroblast Cells" 한국미생물·생명공학회 26 (26): 1224-1233, 2016

13 E. Fuchs, "Epidermal differentiation and keratin gene expression" 17 : 197-, 1993

14 J. Ahn, "Eleutheroside E, an active component of Eleutherococcus senticosus, ameliorates insulin resistance in type 2 diabetic db/db mice" 2013 : 2013

15 C. He, "Eleutheroside E ameliorates arthritis severity in collageninduced arthritis mice model by suppressing inflammatory cytokine release" 37 (37): 1533-, 2014

16 A. Arouca, "Eleutherococcus senticosus: studies and effects" 2013 : 2013

17 Y. B. Kwon, "Effects of c olostrum on keratinocyte differentiation and wound healing" 14 (14): 45-, 2007

18 G. N. Benzdetko, "Effect of Eleutherococcus glycosides on nuclear activity of RNA-polymerase in skeletal muscle and liver after physical load" 19 : 245-, 1973

19 E. J. Shin, "Caffeic acid phenethyl ester inhibits UV-induced MMP-1 expression by targeting histone acetyltransferases in human skin" 20 (20): 3055-, 2019

20 M. Naveed, "C hlorogenic a cid(CGA) : A pharmacological review and call for further research" 97 : 67-, 2018

21 M. S. Blois, "Antioxidant determinations by the use of a stable free radical" 181 (181): 1199-, 1958

22 I. I. Brekhman, "Ancient ginseng and pharmacology of the future" 9 : 1975

23 C. Wei, "Acanthoic acid inhibits LPS-induced inflammatory response in human gingival fibroblasts" 38 (38): 896-, 2015

24 H. Tominaga, "A water-soluble tetrazolium salt useful for colorimetric cell viability assay" 36 (36): 47-, 1999