Hepato-protective effect of fucoidan extracted from acid-processed Sargassum fusiformis in ethanol-treated Chang liver cells and in a zebrafish model

다국어 초록 (Multilingual Abstract)

In our previous study, the anticancer effect of the active fucoidan (JHCF4) isolated from acid-processed Sargassum fusiformis was evaluated. In this study, the liver-protective effects of JHCF4 against ethanol-induced Chang liver cell damage and apoptosis-related responses were investigated. Furthermore, the low cytotoxicity and high cell viability of JHCF4 against ethanol-induced cell damage, as well as its protective effect against ethanol-induced cell apoptosis, were observed via nuclear staining with Hoechst 33342 in Chang liver cells. Additionally, the treatment of the 72 h post-fertilization (hpf) zebrafish model with JHCF4 increased the ethanol-stimulated survival rates as well as decreased oxidative stress, lipid peroxidation, and cell death levels. JHCF4 was found to significantly decrease steatosis production in the 128 hpf zebrafish model by Oil Red O staining, as well as attenuate the malondialdehyde and increase the glutathione contents, compared with the untreated group. These results demonstrate that JHCF4 has a potential hepato-protective effect against ethanol-induced damage both in vitro and in vivo.

목차 (Table of Contents)

• Abstract
• Introduction
• Materials and methods
• Extraction and separation of JHCF4 from Sargassumfusiformis
• Cell culture
• Protective effect of JHCF4 against ethanol-inducedcellular damage
• Nuclear staining with Hoechst 33342
• Apoptosis analysis by flow cytometry
• Western blot analysis
• Analysis of hepatic oxidative stress in ethanol-treatedzebrafish embryos (72 hpf)
• Measurement of steatosis, malondialdehyde, andglutathione content in ethanol-treated zebrafish larvae(128 hpf)
• Results
• Separation and chemical composition of JHCF4
• Protective effect against ethanol-induced damage inChang liver cells
• Protective effect of JHCF4 against ethanol-treatedzebrafish embryos (72 hpf)
• Protective effect of JHCF4 against ethanol-treatedzebrafish larvae (128 hpf)
• Discussion
• Conclusions
• Compliance with ethical standards
• References