Identification of Grapevine leafroll-associated virus 3 Ampelovirus fromGrapevines in Korea

다국어 초록 (Multilingual Abstract)

Grapevine leaf roll-associated virus 3 (GLRaV-3) is one
of the most important viral diseases of grapevine in the
world. In this study, GLRaV-3 Ampelovirus was identified
from grapevines in Korea by analyzing viral coat
protein size, nucleotide, and amino acid sequences. The
molecular weight of viral coat protein from virusinfected
in vitro plantlets was determined by western
blot using a commercial GLRaV-3 polyclonal antibody.
Western blot analysis showed a coat protein of about 43
kDa. RT-PCR product of about 942 bp which encoded
the coat protein (CP) gene was amplified with specific
primers. When the viruses existed at low titers in the
host plant, the dsRNA had very specific template in RTPCR
amplification of fruit tree viruses. Especially,
small-scale dsRNA extraction method was very reliable
and rapid. Sequence analysis revealed that the CP of the
GLRaV-3 Ko consisted of 942 bp nucleotide, which
encoded 314 amino acid residues. The CP gene of
GLRaV-3 Ko had 98.9% nucleotide sequence and
98.7% amino acid sequence identities with earlier
reported GLRaV-3. This is the first report on molecular
assay of GLRaV-3 Ampelovirus identified from Korea.
The GLRaV-3 Ko CP clone would be very useful for
breeding of virus resistant grapevines.

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