토끼 콩팥에 대한 키토산 미세구의 색전 효과 = Embolization Effects of Chitosan Microspheres on Rabbit Kidney

다국어 초록 (Multilingual Abstract)

The aim of the study was to evaluate the possibility of therapeutic use of chitosan microspheres as a new embolic material. Twenty four New Zealand white rabbits were divided into four groups. Polyvinyl alhocol (PVA) particles (150-250 ㎛ ) was used in group 1. Chitosan microspheres were used in group 2 (150-250 ㎛ ), group 3 (250-355 ㎛) and group 4 (355-500 ㎛) respectively. Arteriography of right kidney was performed using 4 F cobra catheter. Embolization was continued until complete occlusion of the right renal artery was achieved. Catheters were tested whether the lumen is obstructed by embolic materials during and after embolization. One animal was sacrificed in each group on the 1st and 3rd day, 1st, 2nd, 4th, and 8th week respectively. Basic hematologic and blood chemisty were analyzed, and gross and microscopic pathologic findings were observed. Chitosan microspheres were round to oval shape, and had smooth surface and even size under the light microscope. Successful embolizations were achieved in all twenty four rabbits. On gross pathologic examination, all chitosan microsphere groups showed no differences from PVA particle group in renal contraction and discoloration. Histologically chitosan microsphere groups had a higher incidence of vasculitis. but did not show any recanalization in comparison with PVA particle group. Hematologic and blood chemisty showed no significant abnormal change in all groups. In conclusion, effective, technically easy and safe embolizations were achieved with chitosan microspheres in rabbit kidney. Chitosan microspheres might be a good embolic material.