만성 치근단주위 병소조직의Arachidonic acid 대사에 관한 연구 = A STUDY ON ARACHIDONIC ACID METABOLISM OF CHRONIC PERIAPICAL LESIONS

다국어 초록 (Multilingual Abstract)

This study was executed to measure the biosynthesis of arachidonic acid metabolic products in chronic periapical lesions, to compare the products among peripical granuloma, periapical cyst and chronic periapical abxcess, and to understatnd the pathogensis of chronic periapical lesions. Tissues from 33 chronic periapical lesions of human teeth were enucleated during endodontic surgery. large part of each tissue was contained in liquid nitrogen immediately and the other was examined histologically.
In histologically diagnosed 8 cases of periapical granuloma, 9 cases of periapical cyst and 8 cases of chronic periapical absecss, the tissues were homogenated and incubated with _14C-arachidonic acid. Lipid solvent extracts were separated by thin laryer chromatography to be analyzed by autoradiography and TLC anlyzer.
1. TXB₂,6-keto-PGF₁α and PGE₂,LTB₄,HETEs and unidentified product which are metabolic products of arachidonic acid were measured in the tissues of chronic peripaical lesions.
2. In all of periapical granuloma, cyst and abscess, the conversion rate of HETEs among all products was the highest(P<0.05), and the percentage of HETEs in total converted products was also the highest(P<0.05).
3. The concentration of each arachidonic acid product was higher in chronic periapical absecss than in periapical granuloma and cyst(P<0.05). The concentration fo TXB₂and HETEs in periapical cyst were hight than in periapical granuloma.
4. The relative amonuts of total products from lipoxygenase pathway to those from cyclo-oxygenase pathway were about 7 fold in chronic periapical lesions. There was no difference among periapical granuloma, cyst and abscess(P<0.05). The total amonut of procucts from cach pathway were hither in chronic periapical abscess than in periapical cyst and granuloma.