메탄산화균의 메탄 산화속도에 미치는 암모니아의 영향 = Effect of Ammonia on the Oxidation of Methane by Methanotrophs

다국어 초록 (Multilingual Abstract)

Rhizosphere and non-rhizosphere soils were sampled from landfill area, riparian wetland, and rice paddy.
The consortia were obtained by methane enrichment culture using the soils. The effects of ammonia on methane oxidation in the consortia were evaluated. Compared with methane oxidation rates without ammonia, the rates with ammonia of 1mg-N/bottle were similar or slightly lower. However, their methane oxidation rates were significantly reduced with 2~4mg-N ammonia/bottles. The effect of ammonia on the methanotrophic abundance was estimated by using a quantitative real-time PCR method targeting particulate methane monooxygenase gene. Ammonia didn’t negatively influence on the methanotrophic abundance although it inhibited the methane oxidation activity by methanotrophs.

국문 초록 (Abstract)

매립지, 습지 및 논으로부터 근권 및 비근권 토양을 채취하여 메탄으로 농화 배양한 후, 농화배양액의 메탄 산화에 미치는 암모니아 농도의 영향을 정량적으로 분석하였다. 순수 메탄만을 첨가한 조건에서의 메탄 산화속도와비교하여, 암모니아 첨가량이 1mg-N/bottle까지는 메탄 산화속도는 거의 유사하거나 약간 감소하는 경향을 보였다. 그러나, 암모니아 첨가량이 2~4mg-N/bottles인 조건에서는 메탄 산화속도가 급격하게 감소하였다. Particulate methane monooxygenase gene을 이용한 quantitative real-time PCR 기법을 활용하여 메탄산화균에 미치는 암모니아 영향을 정량적으로 분석한 결과, 메탄산화균의 군집 밀도는 암모니아 첨가에 의해 감소되지 않았다.

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