The FvCYP714C2 gene plays an important role in gibberellin synthesis in the woodland strawberry

다국어 초록 (Multilingual Abstract)

Background Fragaria vesca, the woodland strawberry, is a diploid relative of the cultivated strawberry. A GA-defcient mutant was found in ethyl methanesulfonate (EMS)-mutagenized lines of the Fragaria vesca accession ‘Yellow Wonder’.
Objective CYP714C2 was found to be diferentially expressed using RNA-seq analysis. It is necessary to identify the function of this gene.
Methods In order to identify the function of this gene, it was cloned and transformed into Arabidopsis thaliana.
Results The DNA sequence of CYP714C2 was found to be 1940 bp in length, with an open reading frame (ORF) of 1539 bp that is predicted to encode a protein of 512 amino acids. The hydrophilicity of this protein is low and it is unstable. The highest relative expression of FvCYP714C2 was found in the leaves, followed by the pedicels, and low expression levels were found in the other tissues examined. Constitutive expression of FvCYP714C2 signifcantly promoted the growth of transgenic A. thaliana plants; transgenic Arabidopsis plants grew faster and grew well than wild type Col-0 plants. GA1+3 contents of the genetically modifed Arabidopsis lines were signifcantly higher than that in the wild type.
Conclusion We conclude that FvCYP714C2 is a gene that functions in the gibberellin biosynthesis pathway in strawberry.

참고문헌 (Reference)

1 Yingying Zhang, "Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation" Wiley 67 (67): 342-353, 2011

2 Saitou N, "The neighbor-joining method: a new method for reconstructing phylogenetic trees." Oxford University Press (OUP) 4 : 406-425, 1987

3 Vladimir Shulaev, "The genome of woodland strawberry (Fragaria vesca)" Springer Science and Business Media LLC 43 (43): 109-116, 2011

4 Tetsuo Kushiro, "The Arabidopsis cytochrome P450 CYP707A encodes ABA 8′-hydroxylases: key enzymes in ABA catabolism" Wiley 23 (23): 1647-1656, 2004

5 K. Tamura, "Prospects for inferring very large phylogenies by using the neighbor-joining method" Proceedings of the National Academy of Sciences 101 (101): 11030-11035, 2004

6 David B. Duncan, "Multiple Range and Multiple F Tests" JSTOR 11 (11): 1-42, 1955

7 Li ZG, "Molecular cloning and expression analysis of a cytochrome P450 gene in tomato" 61 : 297-304, 2010

8 Nei M, "Molecular Evolution and Phylogenetics" Oxford University Press 2000

9 Koichiro Tamura, "MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0" Oxford University Press (OUP) 30 (30): 2725-2729, 2013

10 Rho I, "Interspecifc hybridization of diploids and octoploids in strawberry" 134 : 46-52, 2012

11 Masaharu Mizutani, "Impacts of Diversification of Cytochrome P450 on Plant Metabolism" Pharmaceutical Society of Japan 35 (35): 824-832, 2012

12 Yoshikazu Tanaka, "Flower colour and cytochromes P450" Springer Science and Business Media LLC 5 (5): 283-291, 2006

13 Fei CAO, "Expression and functional analysis of FaPH01;H9 gene of strawberry ( Fragariaxananassa )" Elsevier BV 16 (16): 580-590, 2017

14 Yongyou Zhu, "ELONGATED UPPERMOST INTERNODE Encodes a Cytochrome P450 Monooxygenase That Epoxidizes Gibberellins in a Novel Deactivation Reaction in Rice" Oxford University Press (OUP) 18 (18): 442-456, 2006

15 L. Chang, "Detection of Strawberry RNA and DNA Viruses by RT-PCR Using Total Nucleic Acid as a Template" Wiley 155 (155): 431-436, 2007

16 Ehlting J, "Cytochromes P450 in phenolic metabolism" 5 : 239-270, 2006

17 Logacheva MD, "Confdence limits on phylogenies: an approach using the bootstrap" 270 : 783-791, 2008

18 Shou-ming WANG, "Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry ( Fragaria vesca ) using RNA-Seq" Elsevier BV 16 (16): 828-844, 2017

19 David R. Nelson, "Comparative Genomics of Rice and Arabidopsis. Analysis of 727 Cytochrome P450 Genes and Pseudogenes from a Monocot and a Dicot  " Oxford University Press (OUP) 135 (135): 756-772, 2004

20 Toshiyuki OHNISHI, "CYP724B2 and CYP90B3 Function in the Early C-22 Hydroxylation Steps of Brassinosteroid Biosynthetic Pathway in Tomato" Oxford University Press (OUP) 70 (70): 2071-2080, 2006

21 Kentaro Takei, "Arabidopsis CYP735A1 and CYP735A2 Encode Cytokinin Hydroxylases That Catalyze the Biosynthesis of trans-Zeatin" Elsevier BV 279 (279): 41866-41872, 2004

22 Shigeki Saito, "Arabidopsis CYP707As Encode (+)-Abscisic Acid 8′-Hydroxylase, a Key Enzyme in the Oxidative Catabolism of Abscisic Acid" Oxford University Press (OUP) 134 (134): 1439-1449, 2004

23 Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method" 25 : 402-408, 2001

24 Rzhetsky A, "A Simple Method for Estimating and Testing Minimum-Evolution Trees" Oxford University Press (OUP) 9 : 945-967, 1992

25 David Nelson, "A P450-centric view of plant evolution" Wiley 66 (66): 194-211, 2011

26 Hiroshi Magome, "CYP714B1 and CYP714B2 encode gibberellin 13-oxidases that reduce gibberellin activity in rice" Proceedings of the National Academy of Sciences 110 (110): 1947-1952, 2013