柚子(Citrus junos Siebet Tanaka)의 生長點 培養에 關한 硏究 = Studies on the Meristem Culture in YuZu(Citrus junos Siebet Tanaka) in Vitro

다국어 초록 (Multilingual Abstract)

Studies were conducted to establish the method of sterilization of shoots, to select optimum time for shoot collection, to determine concentration of growth regulators for the callus induction and platelet from the callus of Yuzu.
These works were parts of the attempts to explore the possibility obtaining the virus-free platelets through the meristem culture.
The results obtained were summarized as follows.
Yuzu shoots were readily damaged by common surface sterilants.
In this study, the following procedure provided undamaged sterile shoot tips: the terminal 3-5㎝ portions of the shoots were collected into sterile distilled water and then immersed momentarily in 0.04% Tween 80 and 3-4 washes of sterile distilled water and immediately afterwards in 0.04% Tween 20 and 0.5% Sodium hypochlorite solution for 10-15 minutes, then followed by a rinse of 95% ethanol and 3-5 washed of sterile distilled water.
The optimum time for shoot collection was many.
The best medium for the callus induction was the modified MS medium supplemented with 2.0㎎/ℓIBA, 2.0㎎/ℓ Kinetin, 2.0㎎/ℓ NAA.
The best effective concentration growth regulators for shoot differentiation was IBA 1.0㎎/ℓ+IBA 1.0㎎/ℓ+GA_3 0.1㎎/ℓ
The best effective concentration of growth regulators for root differentiation was IBA 1.0㎎/ℓ+GA_3 0.1㎎/ℓ or IBA 1.0㎎/ℓ