Norflurazon causes developmental defects including cardiovascular abnormalities in earlystage zebrafish (Danio rerio )

다국어 초록 (Multilingual Abstract)

Norflurazon is widely used on agricultural lands and has a high potential to pollute water sources. However, its effects on fish have not been fully elucidated.
The purpose of our study was to determine whether norflurazon adversely affects the developmental stage of zebrafish, which are frequently used as a model system to evaluate the environmental impact of pollutants. Norflurazon interfered with the hatching of zebrafish embryos and induced several sublethal deformities including body length reduction, increased yolk sac volume, and enlargement of the pericardial region. We further examined the cardiotoxicity of norflurazon in the flk1:eGFP transgenic zebrafish line. The vascular network, mainly in the brain region, was significantly disrupted in norflurazon-exposed zebrafish. In addition, due to the failure of cardiac looping, norflurazon-exposed zebrafish had an abnormal cardiac structure. These developmental abnormalities were related to the apoptotic process triggered by norflurazon. Overall, the present study demonstrated the non-target toxicity of norflurazon by analyzing the hazardous effects of norflurazon on developing zebrafish.

참고문헌 (Reference)

1 Birke A, "Zebrafish embryo and acute fish toxicity test show similar sensitivity for narcotic compounds" 36 : 131-135, 2019

2 D’Costa A, "Zebrafish development and genetics : introducing undergraduates to developmental biology and genetics in a large introductory laboratory class" 6 : 169-177, 2009

3 Zoupa M, "Zebrafish as an alternative vertebrate model for investigating developmental toxicity-the triadimefon example" 18 : 817-, 2017

4 Hill AJ, "Zebrafish as a model vertebrate for investigating chemical toxicity" 86 : 6-19, 2005

5 Sant KE, "Zebrafish as a model for toxicological perturbation of yolk and nutrition in the early embryo" 5 : 125-133, 2018

6 Tessadori F, "Twisting of the zebrafish heart tube during cardiac looping is a tbx5-dependent and tissue-intrinsic process" 10 : e61733-, 2021

7 Horvat T, "Toxicity testing of herbicide norflurazon on an aquatic bioindicator species--the planarian Polycelis felina (Daly.)" 73 : 342-352, 2005

8 Küçükoğlu M, "The effects of zinc chloride during early embryonic development in zebrafish(Brachydanio rerio)" 37 : 158-164, 2013

9 Sathishkumar P, "Sonophotocatalytic mineralization of Norflurazon in aqueous environment" 146 : 216-225, 2016

10 Munn MD, "Pesticide Toxicity Index for Freshwater Aquatic Organisms" U.S. Department of the Interior, U.S. Geological Survey 34-, 2001

11 Busquet F, "OECD validation study to assess intra-and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing" 69 : 496-511, 2014

12 Wilson PC, "Norflurazon and simazine losses in surface runoffwater from flatwoods citrus production areas" 78 : 341-344, 2007

13 Desgrange A, "Left-right asymmetry in heart development and disease: forming the right loop" 145 : 162776-, 2018

14 박하현 ; 송권화 ; 임화선, "Isoprocarb induces acute toxicity in developing zebrafish embryos through vascular malformation" 사단법인 한국동물생명공학회 36 (36): 17-24, 2021

15 Wilson PC, "Influence of exposure concentration and duration on effects and recovery of Lemna minor exposed to the herbicide norflurazon" 64 : 228-234, 2013

16 Smirnova A, "Increased apoptosis, reduced Wnt/β-catenin signaling, and altered tail development in zebrafish embryos exposed to a human-relevant chemical mixture" 264 (264): 128467-, 2021

17 Chen J, "Impaired cardiovascular function caused by different stressors elicits a common pathological and transcriptional response in zebrafish embryos" 10 : 389-400, 2013

18 Huang S, "Imazalil and its metabolite imazalil-M caused developmental toxicity in zebrafish(Danio rerio)embryos via cell apoptosis mediated by metabolic disorders" 184 : 105113-, 2022

19 Wang Q, "Identification of apoptosis and macrophage migration events in paraquat-induced oxidative stress using a zebrafish model" 157 : 116-124, 2016

20 하유나 ; 김영진 ; Choi Jeongyeon ; 황인철 ; Ko Ju-Young ; 전희경 ; 김연정, "Evaluation of cytotoxicity, genotoxicity, and zebrafish embryo toxicity of mixtures containing Hyssopus officinalis, Morus alba, Engraulis japonicus, and 27 other extracts for cosmetic safety assessment" 대한독성 유전단백체 학회 17 (17): 221-232, 2021

21 Parlak V, "Evaluation of apoptosis, oxidative stress responses, AChE activity and body malformations in zebrafish(Danio rerio)embryos exposed to deltamethrin" 207 : 397-403, 2018

22 Glaberman S, "Evaluating the zebrafish embryo toxicity test for pesticide hazard screening" 36 : 1221-1226, 2017

23 Gupta A, "Established, new and emerging concepts in brain vascular development" 12 : 636736-, 2021

24 Horzmann KA, "Developmental toxicity of trichloroethylene in zebrafish(Danio rerio)" 22 : 728-739, 2020

25 안가람 ; 박하현 ; 송권화 ; 임화선, "Developmental toxicity of dimethachlor during zebrafish embryogenesis mediated by apoptosis and oxidative stress" 사단법인 한국동물생명공학회 36 (36): 2-8, 2021

26 Fujita M, "Assembly and patterning of the vascular network of the vertebrate hindbrain" 138 : 1705-1715, 2011

27 Schuler LJ, "Aquatic risk assessment of herbicides in freshwater ecosystems of South Florida" 54 : 571-583, 2008