A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P> <P>Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions <I>via</I> conventional free radical polymerization technique. To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker. The composition and probable structure of the terpolymer (HA-g-pHEA-x-GM) were analysed by FTIR, <SUP>1</SUP>H HR-MAS-NMR, and TGA analyses. The SEM and texture analyses of hydrogel showed interconnected micro-porous network and high mechanical properties, respectively. <I>In vitro</I> biocompatibility was studied against human chondrocytes, whereas, <I>in vivo</I> biocompatibility and tissue regeneration were confirmed using mouse model. The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hyaluronate-based terpolymeric gel was synthesized and physicochemically analysed. </LI> <LI> <I>In vitro</I> cell study showed chondrocytes survival and promotion of cell behaviours. </LI> <LI> <I>In vivo</I> biocompatibility was confirmed using mouse model. </LI> <LI> The hydrogel releases bovine serum albumin and dexamethasone in a sustain way. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>