First Report of Freesia sneak virus in Freesia spp. in Korea

다국어 초록 (Multilingual Abstract)

In March, 2013, twenty symptomatic freesia plants (10 plants of cultivar Shiny Lemon and 10 plants ofcultivar Shiny Gold), with striking virus-like symptoms were collected in Cheongju, Korea. The plantsshowed chlorotic, coalescing, interveinal, whitish, necrotic, mosaic, mottling or dark brown-to-purplenecrotic spots on leaves. Freesia crude sap was directly analyzed by transmission electron microscopy, whichpotyvirus particles as well as long virus-like particles were detected. Total RNA extracts were analyzed for theinfection of Freesia sneak virus (FreSV) by reverse transcription (RT)-PCR with primers specific to FreSVcoat protein (CP) gene based on the sequences of FreSV isolates (GenBank No. GU071089, FJ807730 andDQ885455), showing 9 of 20 plants were infected. All 1305bp RT-PCR products were cloned and sequenced.
Comparisons of nucleotide and deduced amino acid sequences using BLAST and bioinformatics toolsresulted in 99 to 100% sequence identity with FreSV isolates FOV, Virginia, and Italy, confirming FreSV in 9symptomatic freesia plants. Of 9 determined cDNAs of FreSV isolates, sequences of 5 cDNA clones wereidentical (GenBank No. AB811437) and sequences of 4 cDNA clones were identical (GenBank No. AB811792).
To our knowledge, this is the first report of FreSV from Freesia spp. in Korea.

참고문헌 (Reference)

1 김경희, "그린키위와 골드키위 과실무름병 예방약제의 적정 살포시기, 간격 및 횟수" 한국식물병리학회 19 (19): 1-6, 2013

2 Ao, M., "Volatile compound in cut and un-cut flowers of tetraploid Freesia hybrida" 27 : 37-40, 2013

3 Vaira, A. M., "The partial sequence of RNA1 of the ophiovirus Ranunculus white mottle virus indicates its relationship to rhabdoviruses and provides candidate primers for an ophiovirus-specific RT-PCR test" 148 : 1037-1050, 2003

4 Martín, S., "The complete nucleotide sequence of a Spanish isolate of Citrus psorosis virus: comparative analysis with other ophioviruses" 150 : 167-176, 2005

5 Choi, H. I., "The complete genome sequence of Freesia mosaic virus and its relationship to other potyviruses" 155 : 1183-1185, 2010

6 García, M. L., "The closely related citrus ringspot and citrus psorosis viruses have particles of novel filamentous morphology" 75 : 3585-3590, 1994

7 Vaira, A. M., "Ophioviruses infecting ornamentals and a probable new species associated with a severe disease in freesia" 722 : 191-199, 2006

8 Vaira, A. M, "Ophiovirus, Encyclopedia of Virology. Vol. 5" Elsevier 447-454, 2008

9 van der Wilk, F., "Nucleotide sequence of genomic organization of an ophiovierus associated with lettuce big-vein disease" 83 : 2869-2877, 2002

10 Sasaya, T, "Molecular analysis and virus transmission tests place Olpidium virulentus, a vector of Mirafiori lettuce big-vein virus and Tobacco stunt virus, as a distinct species rather than a strain of Olpidium brassicae" 72 : 20-25, 2006

11 Tamura, K., "MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance,and maximum parsimony methods" 28 : 2731-2739, 2011

12 Derks, A. F. L. M., "Leafyellowing in combination with corm necrosis in freesia caused by bean yellow mosaic virus: factors involved in syndrome development" 9 : 159-166, 1987

13 Brunt, A. A., "In virus and virus-like diseases of bulb and flower crops" Jon Wiley & Sons 274-280, 1995

14 Hiraguri, A., "Identification of a movement protein of Mirafiori lettuce big-vein ophiovirus" 94 : 145-1150, 2013

15 Kumar, Y., "Identification and characterization of Bean yellow mosaic virus infecting Freesia" 18 : 253-255, 2009

16 Navarro, J. A., "Genetic variability in the coat protein genes of lettuce big-vein associated with virus and Mirafiori lettuce big-vein virus" 150 : 681-694, 2005

17 Sasaya, T., "Further evidence of Mirafiori lettuce big-vein virus but not of Lettuce big-vein associated virus with big-vein disease in lettuce" 98 : 464-468, 2008

18 Vaira, A. M., "First report of Freesia sneak virus infecting Lachenalia cultivars in South Africa" 91 : 770-, 2007

19 Vaira, A. M., "First report of Freesia sneak virus in Freesia sp. In Virginia" 93 : 965-, 2009

20 Vaira, A. M., "Family Ophioviridae, In Virus Taxonomy: Classification and Nomenclature. Ninth Report of the International Commitee on Taxonomy of Viruses" Academic Press 743-748, 2011

21 Naum-Onganía, G., "Citrus psorosis virus RNA1 is of negative polarity and potentially encodes in its complementary strand a 24 K protein of unknown function and 280 K putative RNA dependent RNA polymerase" 96 : 49-61, 2003

22 Roggero, P., "An Ophiovirus isolated from lettuce with big-vein symptoms" 145 : 2629-2642, 2000

23 Verbeek, M., "An Ophiovirus isolated from freesia with freesia leaf necrosis disease, In 11th ISVDOP, Taichung, Taiwan. Wang, L. 2006. Freesia, Flower Breeding and Genetics" Springer 665-693, 2006