서해산 일반 꽃게와 보라색 변이 꽃게의 계측형질 및 AFLP 분석에 의한 집단 구조 = Population Structure of the Blue and Purple Types of the Blue Crab Portunus trituberculatus (Miers) from the West Sea of Korea Based on Morphometric Characteristics and AFLP Analysis

다국어 초록 (Multilingual Abstract)

In Korean waters, there are two color types (blue and purple) of the blue crab Portunus trituberculatus. The blue type is common, but the ratio of the purple type has increased in landings. To determine whether there were significant morphometric or genetic differences between the blue and purple types, crabs caught from the West Sea of Korea were examined. Based on covariance analysis, there were significant differences in 1 of 10 morphometric characteristics of males between the two types, in none of the ten characteristics for females. Using amplified fragment length polymorphism (AFLP) DNA fingerprinting, no specific AFLP marker was detected for each type. The heterozygosity and genetic diversity were very low. Analyses of pairwise distance, the Fst index, and genetic similarity revealed similar results, with very low genetic differentiation. Therefore, there is no significant difference between blue and purple types of the crab from the West Sea of Korea, and the two types in the West Sea can be managed as one stock.

참고문헌 (Reference)

1 성용길, "대하 Fenneropenaeus chinensis 집단의 AFLP 지문에 의한 유전 다양성 및 변이" 한국양식학회 20 (20): 255-259, 2007

2 Miller,M, "Tools for population genetic analysis (TFPGA) 1.3: a windows programme for the sanalysis of allozyme and molecular population genetic data"

3 Wright, S, "The genetic structure of populations" 15 : 313-354, 1951

4 Li, Z., "The effects of selective breeding on the genetic structure of shrimp Fenneropenaeus chinensis populations" 258 : 278-282, 2006

5 Carlson,S.P, "The color changes in Uca pugilator" 21 : 549-551, 1935

6 Sakai,T, "Studies on the Crabs of Japan. IV. Brachygnatha. Brachyrhyncha. Sci. Rep. Tokyo Bunrika Daigaku, Sect. 2B"

7 Bonin, A., "Statistical analysis of amplified fragment length polymorphism data: a toolbox for molecular ecologists and evolutionists" 16 : 3737-3758, 2007

8 MOMAF, "Statistical Yearbook of Maritime Affair and Fisheries 2003-2207" Ministry of Maritime and Fisheries, Korea 2207

9 Snedecar, G.W, "Statistical Method" Iowa State University Press, Ames, IO, USA 1-507, 1980

10 Reineke, A, "Simplified AFLP protocol: replacement of primer labeling by the incorporation of alpha-labeled nucleotides during PCR" 28 : 622-623, 2000

11 McGaw, I.J, "Salinity preference of the shore crab, Carcinus maenas in relation to coloration during intermoult and to prior acclimation" 155 : 145-159, 1992

12 Jones, C.J., "Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories" 3 : 381-390, 1997

13 Huxley,J, "Problems of Relative Growth" London, UK 1-276, 1932

14 Kim, Y.M, "On the result of morphological measurement of the red sea bream, Pagrus major in the southern and western coast of Korea, Rep" 8 : 103-111, 1970

15 Park, C.S, "Morphormetric comparison of herring, Clupea pallasi Cuvier et Valenciennes, between western waters and eastern waters of Korea" 27 : 103-109, 1981

16 Nei, M, "Mathematical model for studing genetical variation in terms of restriction endonucleases" USA 74 : 5267-5273, 1979

17 Mackill, D.J., "Level of polymorphism and genetic mapping of AFLP markers in rice" 39 : 969-977, 1996

18 Kim, I.H, "Korean barnacles (Crustaces, Cirriprdia, Thoracia)" Seoul National University 1985

19 Aarts, H.J., "High- resolution genotyping of Salmonella strains by AFLP-fingerprinting" 26 : 131-135, 1998

20 Mariette, S., "Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite marker" 86 : 469-479, 2001

21 Felder, D.L, "Genetic differentiation in trans-Floridian species complexes of Sesarma and Uca (Decapoda: Brachyura)" 14 : 191-209, 1994

22 Yeon, I.J, "Fishery biology of the Blue crab, Portunus trituberculatus (Miers) in the West Sea of Korea and the East China Sea" Thesis, Pukyong National University, Korea, 1-139 1997

23 Zhang, C.I, "Fisheries Resources Ecolog" Wooseong Co., Seoul, Korea 1-399, 1991

24 Cushing,D.H, "Fisheries Biology, A Study in Population Dynamics" University of Wisconsin Press, Madison, WI, USA 1-200, 1968

25 Melville-Smith, R., "Factors affecting colour change in 'white' western rock lobsters, Panulirus cygnus" 291 : 111-129, 2003

26 Rusell, J.R., "Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs, and RAPDs" 95 : 714-722, 1997

27 Yeon, I.J., "Compara- tive morphometric characteristics and commensal barnacles of the blue crab, Portunus trituberculatus (Miers) in the western coast of Korea and the East China Sea" 46 : 53-68, 1992

28 Zar,J.H, "Biostatistical Analysis" Prentice- Hall International, Inc., Upper Saddle River, NJ, USA 1-663, 1999

29 Stefan, S., "Arlequin ver. 2.000: a software for population genetics data analysis" Genetics and Biometry Laboratory, Uni- versity of Geneva, Switzerland 2000

30 Wang, Z.Y., "Applications of AFLP technology in genetic and phylogenetic analysis of penaeid shrimp" 32 : 399-407, 2003

31 Knorr, C., "Application of AFLP markers to genome mapping in poultry.Anim.Genet.,30,28-35" 30 : 28-35, 1999

32 Blears, M.J., "Amplified fragment length polymorphism (AFLP): a review of the procedure and its applications" 21 : 99-114, 1998

33 Vos, P., "AFLP,a new technique for DNA fingerprinting.Nucl.Acids Res.,23,4407-4414" 23 : 4407-4414, 1995

34 Roa, A.C., "AFLP analysis of relationships among cassava and other Manihot species" 95 : 741-746, 1997

35 Kim, H.S, "A Catalog of Anomura and Brachyura from Korea. Encyclopedia of Fauna & Flora of Korea" Seoul, Korea 14 : 1-694, 1973