Regioselective Enzymatic Procedure for Preparing 3'-O-stearoyl-6- azauridine by Using Burkholderia cepacia Lipase

다국어 초록 (Multilingual Abstract)

Previous studies on the lipase-mediated acylation of 6-azauridine with vinyl stearate in organic solvents revealed that while preparing a potential prodrug, 3'-Ostearoyl-6-azauridine, a lipase from Burkholderia cepacia showed high regioselectivity toward the second hydroxyl group. The most suitable reaction solvent, molar ratio of vinyl stearate to 6-azauridine, and reaction temperature were anhydrous acetone, 15:1, and 45°C, respectively. Under these conditions, the initial reaction rate, 3'-regioselectivity,and maximum substrate conversion were as high as 10.4mM/h, 86.0, and 99.0%, respectively.

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