GC-MS에 의한 식용유의 휘발성 산화생성물 분석 = Gas Chromatography-Mass Spectrometric Analysis of Volatiles from Vegetable Oil

다국어 초록 (Multilingual Abstract)

To study the rapid and simple gas chromatographic method evaluating oxidation progress in vegetable oils, volatile compounds of several commercially processed corn, rapeseed and cottonseed oils stored at 60℃ and 70℃ with daily exposure of fluorescent light for 12 hours were analyzed using gas chromatography and gas chromatography-mass spectrometry. For gas chromatographic analysis, the volatile compounds were recovered by porous polymer trap using splitless mode glass liner of GC injection port, filled with 0.07g Tenax GC and flushed into a fused silica capillary column at 250℃. Twenty-three GC peaks including pentanal, hexanal, pentane, 1-pentanol, t-2-hexenal, heptanal, t-2-heptenal and t-2-octenal were identified on the basis of relative retention time of reference compounds and gas chromatography-mass spectrometry. The volatile compounds identified were autoxidation products of principal unsaturated fatty acids of vegetable oils. The stimulus threshold value of pent anal, heptanal and hexanal were 0.007ppm/paraffin, 0.08ppm/paraffin and 0.09ppm/paraffin, respectively. Among 2-alkenals the stimulus threshold value of t-2-hexenal, t-2-heptenal, t-2-octenal, t-2-nonenal, t,t-2,4-heptadienal and t,t,-2,4-decadienal were 0.50ppm/paraffin, 5.00ppm/paraffin, 0.15ppm/water, 0.0009ppm/water, 0.40ppm/water and 0.025ppm/water, respectively.

목차 (Table of Contents)

• ABSTRACT = 41
• Ⅰ. 서론 = 42
• Ⅱ. 실험재료 및 방법 = 42
• 1. 실험 재료 = 42
• 2. 휘발성 산화생성물의 포집 및 용출 = 43
• 3. 휘발성 산화생성물의 분리 및 동정 = 43
• 4. 관능검사에 의한 휘발성 산화생성물의 역치(threshold value) 측정 = 44
• Ⅲ. 결과 및 고찰 = 44
• 1. 지방산 조성 = 44
• 2. 식용유의 휘발성 산화생성물 = 44
• 3. 대두유 휘발성 산화생성물의 역치(threshold value) = 46