In this study, an integrated micro-nanofluidic system for protein analysis was presented. The device is comprised of a micromixer and a preconcentrator with a separation column. The Integrated micromixer based on unbalanced split and cross collision o...
In this study, an integrated micro-nanofluidic system for protein analysis was presented. The device is comprised of a micromixer and a preconcentrator with a separation column. The Integrated micromixer based on unbalanced split and cross collision of fluid streams is passive and planar, which is easy to fabricate and integrate to the microfluidic system. The preconcentrator based on electrokinetic trapping of protein has nanochannels formed by the electrical breakdown of PDMS membrane using high electric shock, without any nano-lithographic process. Micromixer and preconcentrator were used for sample preparation and concentration of protein, consecutively. In sample preparation, proteins were tagged with conjugate dye or other bio marker proteins were electrokinetically trapped at anodic side of the junction of micro/nanochannels. This simple integrated microdevice could be useful for various bioanalysis and biosensor application.