Immobilization of Fibrinogen Antibody on Self-Assembled Gold Monolayers for Immunosensor Applications

다국어 초록 (Multilingual Abstract)

Self-assembled gold monolayers offer several advantages for the realization of novel modified electrodes for biosensor applications. This is due to their ability to decrease non-specific adsorption and provide for covalent attachment of biomolecules. Surfaces for these applications require the precise control of ligand density, the ability to immobilize ligands, and in situ-modulation of ligand activity. In this study, we focused our studies on the immobilization of antibody on a gold monolayer surface. We self-assembled thioctic acid onto the gold surface as an anchor point for the immobilization of anti-fibrinogen onto the surface. The modifications to the gold surface were characterized by ELISA, ellipsometry, and AFM.

참고문헌 (Reference)

1 I Park, "Thiolated salmonella antibody immobilization onto the gold surface of piezoelectric quartz crystal" 13 : 1091-, 1998

2 MJ Ford, "The application of gold surfaces and particles in nanotechnology" 13 : 297-, 2006

3 JC Love, "Self-assembled monolayers of thiolates on metals as a form of nanotechnology" 105 : 1103-, 2005

4 T Wink, "Self-assembled monolayers for biosensors" 122 : 43R-, 1997

5 B Lee, "Selective immobilization of biomolecules onto an activated polymeric adlayer" 2 : 136-, 2007

6 SK Vashist, "Quantification of human immunoglobulin G immobilized on gold-coated silicon chip for biosensing applications" 34 : 806-, 2006

7 C Yam, "Preparation, characterization, resistance to protein adsorption, and specific avidin-biotin binding of poly(amidoamine) dendrimers functionalized with oligo(ethylene glycol) on gold" 296 : 118-, 2006

8 GD Lowe, "Plasma fibrinogen" 41 : 430-, 2004

9 LT Banner, "Pinhole-free largegrained atomically smooth Au(111) substrates prepared by flame annealed template stripping" 41 : 49-, 2009

10 D Kim, "Molecular recognition and specific interactions for biosensing applications" 8 : 6605-, 2008

11 S Choi, "Methods of reducing non-specificadsorption in microfluidic biosensors" 20 : 1-, 2010

12 YS Shon, "Low density selfassembled monolayers derived from 2-Monoalkyl-propane-1,3-dithiols" 16 : 541-, 2000

13 K Wadu-Mesthrige, "Immobilization of proteins on self-assembled monolayers" 22 : 380-, 2000

14 W Lee, "Immobilization of antibody fragment for immunosensor application based on surface plasmon resonance" 40 : 143-, 2005

15 JK Lee, "Grafting nitrilotriacetic groups onto carboxylic acid-terminated self-assembled monolayers on gold surfaces for immobilization of histidine-tagged proteins" 108 : 7665-, 2004

16 T Ibii, "Direct immobilization of gold-binding antibody fragments for immunosensor applications" 82 : 4229-, 2010

17 Z Yang, "Development of a biosensor for measuring plasma fibrinogen based on an enzyme kinetic model" 27 (27): 1689-, 2005

18 E Briand, "Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?" 22 : 440-, 2006

19 J. Lahann, "A reversibly switching surface" 299 : 371-, 2003

20 N Backmann, "A label-free immunosensor array using single-chain antibody fragments" 102 : 14587-, 2005

21 BA Snopok, "A biosensor approach to probe the structure and function of the adsorbed proteins: fibrinogen at the gold surface" 1 (1): 121-, 1998

22 M Arruebo, "A Gonzalez-Fernandez, Antibodyconjugated nanoparticles for biomedical applications" 2009 : 1-, 2009