HaCaT Keratinocytes and Primary Epidermal Keratinocytes Have Different Transcriptional Profiles of Cornified Envelope-Associated Genes to T Helper Cell Cytokines = HaCaT Keratinocytes and Primary Epidermal Keratinocytes Have Different Transcriptional Profiles of Cornified Envelope-Associated Genes to T Helper Cell Cytokines

다국어 초록 (Multilingual Abstract)

HaCaT cells are the immortalized human keratinocytes and have been extensively used to study the epidermal homeostasis and its pathophysiology. T helper cells play a role in various chronic dermatological conditions and they can affect skin barrier homeostasis. To evaluate whether HaCaT cells can be used as a model cell system to study abnormal skin barrier development in various dermatologic diseases, we analyzed the gene expression profl le of epidermal differentiation markers of HaCaT cells in response to major T helper (Th) cell cytokines, such as IFN , IL-4, IL-17A and IL-22. The gene transcriptional profl le of cornifl ed envelope-associated proteins, such as fl laggrin, loricrin, involucrin and keratin 10 (KRT10), in HaCaT cells was generally different from that in normal human keratinocytes (NHKs). This suggests that HaCaT cells have a limitation as a model system to study the pathophysiological mechanism associated with the Th cell cytokine-dependent changes in cornifl ed envelope-associated proteins which are essential for normal skin barrier development. In contrast, the gene transcription profl le change of human 2- defensin (HBD2) in response to IFN , IL-4 or IL-17A in HaCaT cells was consistent with the expression pattern of NHKs. IFN also up-regulated transglutaminase 2 (TGM2) gene transcription in both HaCaT cells and NHKs. As an alternative cell culture system for NHKs, HaCaT cells can be used to study molecular mechanisms associated with abnormal HBD2 and TGM2 expression in response to IFN , IL-4 or IL-17A.

참고문헌 (Reference)

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