四君子湯이 免疫機能에 미치는 影響 = Effect of Sagunja-tang on Immune Function of Mouse Immune Cells

다국어 초록 (Multilingual Abstract)

Objectives : The extract of Sagunja-tang has been traditionally used for restorative treatment of constitutional weakness, vascular and immune disorder, and nervous disease in Oriental country. This study investigated the regulatory effects of Sagunja-tang on the expression, production, and activity of immune mediators.Methods : In this study, the extract of Sagunja-tang was prepared by extracting with distilled water at 100$^{\circ}C$ for 2.5h. The extract was freeze-dried following filtration through 0.45${{\mu}m}$ filter. The extract was dissolved in Hank's balanced salt solution (HBSS) and filtered again through 0.45${{\mu}m}$ filter before use. The level of nitrite, an oxidative product of nitric oxide(NO) was measured in the culture medium by the Griess reaction. The levels of prostaglandin E₂(PGE₂), Th1 cytokines (IFN-${\gamma}$, IL-2) and Th2 cytokines(IL-4, IL-5, IL-13) were measured by enzyme-linked immunosorbent assay and the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were determined by Western blot analysis. Also examined the effects of the extract on T-cell proliferation and cytotoxic activity of natural killer cells.Results : In this investigation, Production levels of Th2 cytokines (IL-4, IL-5, IL-13) was inhibited in a dose dependent manner by treatment with the extract. I also found that the extract increased T-cell proliferation and cytotoxic activity of natural killer cells in a dose-dependent manner.Conculsions : These results suggest that the water extract of Sagunja-tang may be useful for a therapeutic drug against a sickly constitution and immune diseases, probably by regulating the production of immune mediators.

참고문헌 (Reference)

1 金度淳, "六味地黃湯, 四物湯, 四君子湯의 항Stress효과에 관한 실험적 비교연구" 慶熙大學校 大學院 1995

2 金聖勳, "사군자탕,사물탕 및 팔물탕이 prednisolone으로 유발된 생쥐의 면역반응저하에 미치는 영향" 慶熙大學校 大學院 1987

3 정우영, "사군자탕 및 사군자탕가반묘가 위암세포에 미치는 영향" 경희대학교 대학원 2001

4 姜政秀, "기아회복시 대사기질, 호르몬, 혈액 성분변화에 미치는 사군자탕, 사물탕 및 팔물탕의 효과" 大田大學校 大學院 1995

5 金建沃, "子宮癌細胞에 대한 楡根皮, 四君子湯, 四君子湯加楡根皮의 抗癌效果" 大田大學校 大學院 1993

6 南容哉, "四君子湯의 效能에 관한 연구" 圓光大學校 大學院 1991

7 裵鍾局, "四君子湯과 四物湯 Extract 투여가 Rat의 성장에 미치는 영향" 경희대학교 대학원 1978

8 李哲浣, "四君子湯,四物湯 및 八物湯이 筋肉疲勞恢復에 미치는 實驗的 硏究" 慶熙大學校 大學院 1989

9 林圭庠, "四君子湯 煎湯液이 家兎의 生體活性에 미치는 影響" 圓光大學校 大學院 1988

10 Ahn YG, "The Effect of Panax Ginseng Extract on the Immunotoxicity of Cimetidine in Mice" 6 (6): 25-38, 1991

11 Jin SM., "Taiping Benevolent Dispensary Bureau" Chinese Medicine Press 18-, 1996

12 Gong YH, "Susebowon" Euiseongdang 227-, 1993

13 Jeong WU, "Sagunja-tang effects on blood pressure and body temperature" Wonkwang University. 1989

14 Ren FY, "Ribavirin and IFN-alpha combination therapy induces CD4+ T-cell proliferation and Th1 cytokine secretion in patients with chronic hepatitis B" 13 (13): 5440-5445, 2007

15 Jeong UC, "Protective Effects of Sagunja-Tang on Oxidative Stress-Induced Death of H9c2Cardiomyoblast Cells" Wonkwang University 2002

16 Pak JY, "Protective Effect of Sagunja-tang on H2O2-induced Oxidative Stress in Human Vascular Endothelial Cells" Wonkwang University 2007

17 Kang SS, "Proper Traditional Medical Formulae" Daeseongmunhwasa 120-121, 1996

18 Yun YG, "Prescriptional survey about crinical application of Sagoonja-tang in the Dongeuibogam" 9 (9): 11-34, 2001

19 Shim JC, "Pharmacological Study on the Effect of Poria and Radix Cynanchi Wilf ordii" Wonkwang University 2002

20 Cho JG, "Oriental medical study on immunity" 12 (12): 19-23, 1986

21 Lee JH, "Nitric oxide inhibits capping in HL-60cells" 232 (232): 827-831, 1997

22 Choi BM, "Nitric oxide as a pro-apoptotic as well as anti-apoptotic modulator" 35 (35): 116-126, 2002

23 Leung DY, "New insights into atopic dermatitis" 113 (113): 651-657, 2004

24 Lee MH, "Internal Medicine" Fraternity Press 1989-1999, 1977

25 Blanco JC, "Interferon regulatory factor(IRF)-1 and IRF-2 regulate interferon gamma-dependent cyclooxygenase 2 expression" 191 (191): 2131-2144, 2000

26 Lee JH, "Illustrated the Chinese side treatment Prescriptions" Uibang publisher 382-384, 2004

27 Weinberg JB, "Human mononuclear phagocyte inducible nitric oxide synthase(iNOS) : analysis of iNOS mRNA, iNOS protein, biopterin, and nitric oxide production by blood monocytes and peritoneal macrophages" 86 (86): 1184-1195, 1995

28 Long M, "Histone acetylation at the Ifng promoter in tolerized CD4 cells is associated with increased IFN-gamma expression during subsequent immunization to the same antigen" 179 (179): 5669-5677, 2007

29 Kim HM, "Herbal Medicinal Pharmacology" Goryeo medicine 315-319, 2000

30 Lee SJ, "Effects of the Ijin-tang, Sagoonja-tang, and Yuggoonja-tang on the Hyperlipidemia induced Rabbits" Wonkwang University 1992

31 Oh JS, "Effects of Korea Ginseng, Deer Horn Extracts and Filtrate of Escherichia coli Culture on Leukocyte Chemotaxis" 20 (20): 247-255, 1985

32 Ha JY, "Effect of Samul-tang and Sagunja-tang on the Intravascular Cagulation Induced by Endotoxin in rats" 11 (11): 113-122, 1988

33 Lee NG, "Effect of SA GUN JA TANG on Immune Response and Natural Killer Cells Activity to SRBC in Mice" 10 (10): 115-122, 1989

34 Yun YG, "East Medical prescription and prescription Commentary" Euiseongdang 330-332, 2002

35 Heo J, "Dongeuibogam" Namsandang 90-, 1969

36 Shin MG, "Clinical herbalogy" Namsandang 250-261, 1986

37 Lee SI, "Clinical application of Chinese medicine" Seongbosa 151-153, 1986

38 Abbas AK, "Cellular and molecular immunology" 3-16, 2000

39 Abbas AK, "Cellular and molecular immunology" Saunders co 235-269, 2000

40 Go BH, "An Experimental Study on the Effects of Deer horn, Rehmannia glutiosa, Panax ginseng and Acanthopanax sessiliflorum on Immune Response & NK Cell Activity in Mice" 9 : 193-216, 1986