Sphingomonas agri sp. nov., a bacterium isolated from soil

다국어 초록 (Multilingual Abstract)

<P> A Gram-reaction-negative, aerobic, non-motile, white (translucent) and rod-shaped bacterium (designated HKS-06<SUP>T</SUP>) isolated from soil was characterized by a polyphasic approach to clarify its taxonomic position. Strain HKS-06T was observed to grow optimally at 30 °C and at pH 6.5-7.0 on R2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HKS-06<SUP>T</SUP> belongs to the genus Sphingomonas and is most closely related to Sphingomonas lutea JS5<SUP>T</SUP> (97.4% similarity). The G+C content of the genomic DNA was 64.1 mol%. Chemotaxonomic data [major quinone (Q-10), major polar lipids (phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, unknown polar lipid) and major fatty acids (summed feature 8, comprising C18 : 1ω7c/ω6c and/or C<SUB>18</SUB> : <SUB>1</SUB>ω6c, C<SUB>18 : 0</SUB> 3-OH and C<SUB>16 : 0</SUB>)] supported the affiliation of strain HKS-06<SUP>T</SUP> to the genus Sphingomonas. Moreover, the physiological and biochemical results and low level of DNA-DNA relatedness [between strain HKS06<SUP>T</SUP> and S. lutea JS5<SUP>T</SUP> (20.24±1.2 %)] allowed the phenotypic and genotypic differentiation of strain HKS-06<SUP>T</SUP> from recognized species of the genus Sphingomonas. The new isolate therefore represents a novel species, for which the name Sphingomonas agri sp. nov. is proposed. The type strain is HKS-06<SUP>T</SUP> (=KACC 18880T=LMG 29563<SUP>T</SUP>). </P>