Effect of Light and Reductones on Differentiation of Pleurotus ostreatus

다국어 초록 (Multilingual Abstract)

Vegetative mycelia of Pleurotus ostreatus were differentiated into primordia and subsequently into fruit bodies in synthetic sucrose-asparagine medium when exposed to light at low temperature. During photomorphogenesis,L-ascorbic acid-like substances called reductones were produced. L-Ascorbic acid, D-erythroascorbic acid, 5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, 5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid, 5-methyl-5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid and 5-methyl-5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid were accumulated initially in the illuminated mycelia before the initiation of fruiting. The content of glycosides of erythroascorbic acid and their methylated compounds increased again in the primordia and the fruit bodies. Exogenous L-ascorbic acid induced the formation of primordia from the mycelia in the dark in a dose-dependent manner. Thus, this suggests that these reductones might play a role in mediating the light stimulus in photomorphogenesis.

참고문헌 (Reference)

1 Zadražil, F, "The ecology and industrial production of Pleurotus ostreatus, Pleurotus florida, Pleurotus cornucopiae and Pleurotus eryngii" 621-652, 1974

2 Eger, G, "The action of light and other factors on the sporophore initiation in Pleurotus ostreatus" 575-583, 1974

3 Liang, Y.T.S, "Synthesis of D-erythroascorbic acid" 9 : 75-84, 1990

4 Allen, R.G, "Superoxide dismutase induces differentiation in microplasmodia of the slime mold Physarum polycephalum" 261 : 205-211, 1988

5 유성운, "Spectral characterization and chemical modification of FMN-containing ascorbyl free-radical reductase from Pleurotus ostreatus" PORTLAND PRESS LTD 341 : 755-763, 1999

6 Hansberg, W, "Reactive oxygen species associated with cell differentiation in Neurospora crassa" 14 : 287-293, 1993

7 Kwon, J.Y, "Purification and characterization of L-gulonolactone oxidase from Pleurotus ostreatus" Seoul National University 1991

8 Szent-Györgyi, A, "Observations on the function of peroxidase systems and the chemistry of the adrenal cortex: description of a new carbohydrate derivative" 22 : 1387-1409, 1928

9 Gonzalez-Reyes, J.A, "Nutrient uptake changes in ascorbate free radical-stimulated onion roots" 104 : 271-276, 1994

10 Vera, J.C, "Mammalian facilitative hexose transporters mediate the transport of dehydroascorbic acid" 364 : 79-82, 1993

11 Seung-Rock Lee, "Intracellular Substrates of a Heme-Containing Ascorbate Oxidase in Pleurotus ostreatus" 한국미생물학회 47 (47): 178-186, 2009

12 Zadražil, F, "Influence of CO2 concentration on the mycelium growth of three Pleurotus ostreatus species" 1 : 327-335, 1975

13 Carlile, M.J, "Inducing sporulation in Alternaria solani. II. Effect of light" 54 : 47-54, 1974

14 Prillinger, H, "Genetic analysis in wood-decaying fungi" 46 : 265-277, 1979

15 Kim, B.N, "Effects of light, L-ascorbic acid and hydrogen peroxide on the differentiation of Pleurotus ostreatus" Seoul National University, 1994

16 Moore-Landecker, E, "Effects of aeration and light on apothecia, sclerotia and mycelial growth in the discomycetes Pyronema domesticum" 74 : 1000-1013, 1982

17 Okamura, M.J, "Distribution of ascorbic acid analogs and associated glycosides in mushrooms" 40 : 81-94, 1994

18 Manachère, G, "Conditions essential for controlled fruiting of macromycetes" 75 : 255-270, 1980

19 Kang, S.O, "A possible enzymatic catabolism of L-ascorbic acid via an ꠑ-ketoaldehyde" 23 : 302-308, 1985

20 유성운, "A heme-containing ascorbate oxidase from Pleurotus ostreatus" AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC 271 : 3105-3111, 1996