인삼의 세포독성분획의 작용기작에 관한 연구 ( 1 ) - 인삼중의 Petroleum Ether 분획이 동물암세포에서 고분자 물질의 합성에 미치는 영향 = The Studies on the mechanism of Action of the Cytotoxic Fraction from Korean Ginseng Roots ( Ⅰ ) . The Effects of Pertroleum Ether Fraction from Korean Ginseng Roots on the Biosynthesis of macromolecules in Mammalian Neoplastic Cells

다국어 초록 (Multilingual Abstract)

The petroleum ether fraction from Korean Ginseng roots has been proved to be cytotoxic to murine lymphocytic leukemia L5178Y cells and murine Sarcoma 180 cells. This petroleum ether fraction which was extracted from Korean ginseng roots inhibited the growth of murine leukemia L5178Y cells and murine Sarcoma 180 cells in vitro, And it also inhibited DNA, RNA and protein synthesis in murine ascitic Sarcoma 180 cells in vitro. The cytotoxicity of this fraction and it`s inhibitory effect on biosynthesis of macromolecules were exponential function of its concentration, The cytotoxicity correlated to the inhibitory effect on the biosynthesis of macromolecules, especially to the inhibitory action on protein synthesis, The inhibitory action of this fraction on the protein synthesis was due to the inhibition of polysome formation: at the concentration which causes 90% inhibition of protein synthesis all the polysomes were dissociated into monosomes, Since messenger RNA degradation was not occurred at this concentration, petroleum ether fraction seems to interfere specifically the initiation of polypeptide synthesis.