Transgenic Rice Expressing Snowdrop Lectin (Galanthus nivalis agglutinin;GNA) Shows Resistance to Rice Brown Planthopper (Nilaparvata lugens Stål)

국문 초록 (Abstract)

유전자총(Particle bombardment) 방법에 의해 snowdrop lectin (Galanthus nivalis agglutinin; GNA) 유전자가 도입된 해충저항성 형질전환 벼를 개발하였다. GNA 유전자가 도입된 많은 형질전환 식물체가 재분화되었으며, 형질전환 벼의 GNA 유전자의 integration, expression 및 inheritance는 Southern 및 western analysis분석 방법에
의해 증명되었다. 벼 genome 내 도입 유전자 수는 one 혹은 five copies이었다. 형질전환 R1과 R2 식물체의 GNA 단백질 발현 분석 결과, 함량은 총 단백질 중 0.01%부터 2.0%까지 포함하고 있었다. 형질전환 식물체 중 GNA과발현 형질전환 벼를 이용한 생물 검정 결과, 대조구와 비교하여 벼의 주요 해충인 벼멸구(Nilaparvata lugens Stal)에 저항성을 나타내었다. 위의 실험 결과를 통해, 해충저항성 유전자인 GNA가 도입된 형질전환 벼는 해충의 증식을 억제하는데 이용될 수 있을 것이다.

다국어 초록 (Multilingual Abstract)

Transgenic rice plants with increased resistance to rice brown planthopper (Nilaparvata lugens Stal) were generated by particle bombardment-mediated transformation of plants with a gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under control of the rice Rubisco small subunit (rbcS) promoter.. A large number of transgenic rice plants containing the GNA gene were generated. The integration, expression, and inheritance of this gene in the R1 and R2 generations were demonstrated by Southern and western blot analyses. The plants contained one to five copies of the transgene. The GNA protein comprised approximately 0.01-2.0% of total soluble protein in the R1 and R2 transgenic plants. Insect bioassays and feeding studies showed that the GNA protein expressed in the R2 transgenic rice plants reduced the survival of brown planthoppers. The introduction of GNA
into rice plants therefore can help to control insect pests.

목차 (Table of Contents)

• Introduction
• Materials and Methods
• Materials and Methods
• References
• Acknowledgement
• 초록

참고문헌 (Reference)

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