<i>Flavobacterium daemonensis</i> sp. nov., isolated from Daemo Mountain soil

다국어 초록 (Multilingual Abstract)

<P>A Gram-staining-negative, catalase- and oxidase-positive, obligately aerobic, motile by gliding, bright yellow and rod-shaped bacterial strain was isolated from soil of Daemo Mountain (Daemosan) in Seoul, Republic of Korea. Its taxonomic position was investigated by using a polyphasic study. On the basis of 16S rRNA gene sequence comparisons, strain THG–DJ7<SUP>T</SUP> was found to be most closely related to <I>Flavobacterium denitrificans</I> ED5<SUP>T</SUP> (97.9 % sequence similarity), <I>Flavobacterium ginsenosidimutans</I> THG 01<SUP>T</SUP> (97.6 %), <I>Flavobacterium kyungheensis</I> THG-107<SUP>T</SUP> (97.5 %), <I>Flavobacterium anhuiense</I> D3<SUP>T</SUP> (97.1 %) and <I>Flavobacterium ginsengisoli</I> DCY54<SUP>T</SUP> (97.0 %). The DNA–DNA relatedness between strain THG–DJ7<SUP>T</SUP> and its phylogenetically closest neighbours was below 40.0 %. The G+C content of the genomic DNA was determined to be 32.6 mol%. The only isoprenoid quinone detected in strain THG–DJ7<SUP>T</SUP> was menaquinone-6 (MK-6). The major component in the polyamine pattern was <I>sym</I>-homospermidine. The major polar lipids were found to be phosphatidylethanolamine, phosphatidyldimethylethanolamine, an unidentified aminophosphoglycolipid, phosphatidylserine and an unidentified lipid. The major fatty acids were identified as iso-C<SUB>15 : 0</SUB>, iso-C<SUB>15 : 0</SUB> 3-OH and summed feature 3 (comprising C<SUB>16 : 1</SUB>ω7<I>c</I> and/or C<SUB>16 : 1</SUB>ω6<I>c</I>). These data support the affiliation of strain THG–DJ7<SUP>T</SUP> to the genus <I>Flavobacterium</I>. We report the phenotypic, genotypic and phylogenetic characterization of strain THG–DJ7<SUP>T</SUP>. Based on the findings presented, a novel species of the genus <I>Flavobacterium</I>, <I>Flavobacterium daemonensis</I> sp. nov. is proposed, with THG–DJ7<SUP>T</SUP> ( = KACC 17651<SUP>T</SUP> = JCM 19455<SUP>T</SUP>) as the type strain.</P>