식물의 저온 적응 메카니즘에서 아스코브산과 관련된 효소 중 MDHA 환원효소의 활성도와 과산화수소, 아스코브산의 함량, mRNA의 발현수준과의 연관성을 연구한 결과는 다음과 같다. MDHA 환원...
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https://www.riss.kr/link?id=A82571584
2011
Korean
KCI등재
학술저널
368-374(7쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
식물의 저온 적응 메카니즘에서 아스코브산과 관련된 효소 중 MDHA 환원효소의 활성도와 과산화수소, 아스코브산의 함량, mRNA의 발현수준과의 연관성을 연구한 결과는 다음과 같다. MDHA 환원...
식물의 저온 적응 메카니즘에서 아스코브산과 관련된 효소 중 MDHA 환원효소의 활성도와 과산화수소, 아스코브산의 함량, mRNA의 발현수준과의 연관성을 연구한 결과는 다음과 같다. MDHA 환원효소의 활성도 변화는 저온에 노출되는 시간이 길어질수록 증가하였으며 6시간 이후에 엽록체분획과 세포질분획에서 급격하게 증가하는 경향을 보였으나 실온으로 회복시켰을 때 효소의 활성도가 상대적으로 감소하는 경향을 보였다. 저온에 노출된 동안 아스코브산의 함량은 비교적 일정한 경향을 보이다가 실온으로 회복시키면 그 이후에는 급격하게 증가하는 경향을 보였다. 반면 저온에 노출되는 동안 급격히 dehydroascorbate 함량이 감소하였다가 실온으로 회복되면 약간 증가하는 경향을 보였다. 아스코브산의 함량과 엽록체분획과 세포질분획의 MDHA 환원효소의 활성도와의 상관관계는 각각 정의 상관(R²=0.9240, 0.9108)을 나타내었으나 디하이드로아스코브산의 함량과 MDHA 환원효소의 활성도 사이에는 각각 부의 상관(R²=0.8638, 0.8980)을 나타내었다. MDHA 환원효소 활성도와 과산화수소의 함량과의 상관관계를 과산화수소의 생성량이 증가하면 MDHA 환원효소의 활성도가 증가하는 정의 상관(R²=0.9443, 0.9647)을 나타내었다. 저온스트레스 처리 시간이 증가할수록 MDHA 환원효소의 mRNA의 발현 수준과 총 MDHA 환원효소의 활성도가 증가하는 경향을 나타내었다.
다국어 초록 (Multilingual Abstract)
The relationship between MDHA reductase activity and ascorbate, dehydroascorbate, and hydrogen peroxide content was evaluated, and this experiment was conducted to determine the change of MDHA reductase activity and the level of steady-state mRNA abun...
The relationship between MDHA reductase activity and ascorbate, dehydroascorbate, and hydrogen peroxide content was evaluated, and this experiment was conducted to determine the change of MDHA reductase activity and the level of steady-state mRNA abundance of MDHA reductase in lettuce leaves subjected to low temperature stress. MDHA reductase activity of chloroplastic and cytosolic fraction in lettuce leaves subjected to 4℃ for 24 hr increased, followed by a steady decrease during the duration of recovery to 20℃ for 48 hr. The content of ascorbate slowly increased during low temperature treatment, followed by a rapid increase during the duration of recovery to 20℃ for 48 hr, while dehydroascorbate content rapidly decreased. The relationship between MDHA reductase activity of chloroplastic and cytosolic fraction in lettuce leaves subjected to 4℃ and ascorbate content correlated positively (R²=0.9240, 0.9108, respectively), but MDHA reductase activity of chloroplastic and cytosolic fraction and dehydroascorbate were reversely correlated (R²=0.8638, 0.8980, respectively). Hydrogen peroxide content and MDHA reductase activity of chloroplastic and cytosolic fraction in lettuce leaves subjected to 4℃ correlated positively (R²=0.9443, 0.9647, respectively). Northern blot analysis showed that the level of mRNA transcript of MDHA reductase was similar to total activity of MDHA reductase, and also that the level of mRNA of MDHA reductase after recovery to 20℃ for 24 hr decreased.
참고문헌 (Reference)
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1 강상재, "저온 처리한 상추의 잎 내에서 ascorbate peroxidase와 dehydroascorbate reductase의 반응" 한국생명과학회 18 (18): 1705-1711, 2008
2 강상재, "수분 부족 스트레스 처리시 Monodehydroascorbate Reductase (MDHAR)의 반응" 한국생물환경조절학회 17 (17): 273-282, 2008
3 Alexieva, V., "The effect of drought and ultraviolet radiation on the growth and stress markers in pea and wheat" 24 : 1337-1344, 2001
4 Shigeoka, S., "Regulation and function of ascorbate peroxidase isoenzymes" 53 : 1305-1319, 2002
5 Sano, S., "Purification and cDNA cloning of chloroplastic monodehydroascorbate reductase from spinach" 69 : 762-772, 2005
6 Asada,K, "Production and action of oxygen species in photosynthetic tissues, In Causes of photooxidative stress and amelioration of defense systems in plants" CRC press Boca Raton 77-104, 1994
7 Leterrier, M., "Peroxisomal monodehydroascorbate reductase. genomic clone characterization and functional analysis under environmental stress conditions" 138 : 2111-2123, 2005
8 Hossain, M. A., "Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide" 25 : 385-395, 1984
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TBTCl (tri-n-butyltin chloride)과 bisphenol A에 의한 단기노출이 등줄쥐의 번식에 미치는 영향
전립선암 세포주인 PC-3에서 cordycepin에 의해 유도된 세포 내 칼슘농도 변화와 미토콘드리아 기능 상실을 통한 세포사멸 유도
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2027 | 평가예정 | 재인증평가 신청대상 (재인증) | |
2021-01-01 | 평가 | 등재학술지 유지 (재인증) | |
2018-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2015-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2011-08-03 | 학술지명변경 | 외국어명 : Korean Journal of Life Science -> Journal of Life Science | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2003-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2001-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.37 | 0.37 | 0.42 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.43 | 0.43 | 0.774 | 0.09 |