Molecular Detection and Analysis of Sweet potato feathery mottle virus from Root and Leaf Tissues of Cultivated Sweet Potato Plants

다국어 초록 (Multilingual Abstract)

identified
as Li-CMV, was isolated from a diseased Korean
native lily (Lilium tsingtauense Gilg). Biological and
serological properties of Li-CMV were characterized,
and reverse transcription-polymerase chain reaction
(RT-PCR) analysis, restriction enzyme profiling of RTPCR
products, and nucleotide sequence analysis of
RNA3 of the virus were performed in this study.
Remarkable differences in symptoms between Li-CMV
and ordinary CMV strains were found in tobacco plants
and Datura stramonium. Li-CMV-infected tobacco
plants (cv. Xanthi-nc and cv. Samsun) induced chlorotic
ringspots on uninoculated upper leaves, and the symptom
expression was delayed or faint, whereas, ordinary
CMV strains induced green mosaic symptoms on the
plant. Systemic infections were observed on Nicotiana
benthamiana with severe mosaic symptom. Restriction
mapping analysis of RT-PCR products using MspI
showed that Li-CMV belonged to CMV subgroup I. A
full-length cDNA copy of RNA3 for the virus was amplified
by RT-PCR, cloned, and its complete nucleotide
sequence was determined. The RNA3 of Li-CMV was
2,232 nucleotides long, and consisted of two open reading
frames of 843 and 657 bases encoding 3a protein
(movement protein) and coat protein, respectively.
Results of this study indicate that Li-CMV is a novel
strain and belongs to subgroup I of CMV in the genus
Cucumovirus.