인천지역 닭 도축장에서 분리된 Salmonella spp.의 항생제 내성 및 PFGE 패턴분석 = Analysis of antimicrobial resistance and PFGE patterns of Salmonella spp. isolated from chickens at slaughterhouse in Incheon area

다국어 초록 (Multilingual Abstract)

Salmonella spp. are the important pathogens both economically and clinically in animals as well human. Some of them have highly zoonotic potentials even though they are asymptomatic in animals. Therefore, the prevalence of Salmonella spp. in animals is highly concerned for human health. The present study was carried out to investigate the prevalence, antimicrobial resistance and PFGE patterns of Salmonella spp. isolated from chickens at slaughterhouse in Incheon area. Thirty seven isolates of Salmonella spp. belonging to 5 serotypes (S. Enteritidis 46.0%, S. Newport 40.5%, S. Typhimurium 8.1%, S. Gallinarum 2.7%, S. Derby 2.7%) were identified with an overall prevalence rate of 7.3 percent. Resistance to nalidixic acid was found in 97.3% of Salmonella spp. isolated, followed by streptomycin (16.2%), tetracycline (16.2%), ampicillin (5.4%). Only 6 isolates (16.2%) showed resistance to more than two antimicrobials. In PFGE analysis of chicken and human isolates with Xba I, S. Enteritidis isolates from chicken showed very high similarity with higher than 82.8% and also the similarity was very high in the comparison with human isolates. However, the higher similarity (100%) was observed only chicken isolates of S. Typhimurium. These results suggest the close genetic relatedness of Salmonella spp. isolated from chickens with human.

참고문헌 (Reference)

1 국립수의과학검역원, "축산물의 가공기준 및 성분규격"

2 박종명, "축산 및 수산분야의 항생물질 사용실태 조사" 27 (27): 205-217, 2003

3 황원무, "인천지역 도축장에서 생산된 닭고기의 미생물 오염도 조사" 28 (28): 59-65, 2004

4 축산물HACCP기준원, "육계사육단계 HACCP 운용지침 및 적용모델"

5 우용구, "우리나라의 가금과 환경에서 분리한 Salmonella species의 특성" 40 (40): 505-514, 2000

6 축산물HACCP기준원, "산란계사육단계 HACCP 운용지침 및 적용모델"

7 성명숙, "도계과정에서의 Salmonella 속 균의 오염에 대하여" 26 (26): 195-205, 2002

8 윤가리, "대구지역 야생조류로부터 분리한 Salmonella속 균의 생물화학적 특성과 Plasmid Profile" 27 (27): 59-67, 2003

9 김능희, "대구지역 도계장에서 처리된 도계육의 Salmonella 오염에 관하여" 22 (22): 365-372, 1998

10 이호원, "닭도체에서 분리한 Salmonella spp의 특성 분석" 한국가축위생학회 30 (30): 339-351, 2007

11 이영주, "닭 분변유래 E.coli 및 Salmonella spp.의 항생제 내성패턴" 대한수의학회 45 (45): 75-83, 2005

12 정석찬, "국내 도축장에서 분리한 세균의 항생제 감수성 조사(2. 도축장의 식육으로부터 분리한 Salmonella균의 항생제 감수성)" 한국수의공중보건학회 31 (31): 51-56, 2007

13 김규태, "경북지역 가축에서 분리한 Salmonella 속균의 혈청형 분포 및 약제 감수성" 27 (27): 47-52, 2003

14 김상윤, "경북지역 가축에서 분리된 Salmonella Typhimurium과 S. Enteritidis의 phage typing 및 pulsed-field gel electrophoresis" 24 (24): 243-253, 2001

15 김상윤, "경북지역 가축에서 Salmonella속 균 감염증에 대한 역학적 특성" 24 (24): 51-68, 2001

16 김규태, "경북서부지방 가축에서 분리된 Salmonella 속균의 생물화학적 특성 및 혈청형" 25 (25): 259-273, 2002

17 국립수의과학검역원, "가축 및 식육 중 항생제 내성균 검사요령"

18 박경윤, "가금류에서 분리한 Salmonella속균의 특성" 18 : 107-116, 1994

19 National Veterinary Assay Laboratory, "The Japanese Veterinary Antimicrobial Resistance Monitoring System.Ministry of Agriculture"

20 European Food Safety Authority, "The Community Summary Report on Trends and Source of Zoonoses,Zoonotic Agents, Antimicrobial resistance and Foodborne outbreaks in the European Union in 2006" 2007

21 Michael L, "Surveillance for Foodborne-Disease Outbreaks-United States(1998-2002)"

22 Garaizar J, "Suitability of PCR fingerprinting, infrequent-restriction-site PCR and pulsed field gel electro-phoresis,combined with computerized gel analysis, in library typing of Salmonella enterica serovar Enteri-tidis" 66 (66): 5273-5281, 2000

23 Henzler DJ, "Salmonella Enteritidis in eggs from commercial chicken layer flocks implicated in human outbreaks" 38 : 37-43, 1994

24 Poppe C, "Salmonella Enteritidis and other Salmonella in laying hens and eggs from flocks with Salmonella in their environment" 56 : 226-232, 1992

25 질병관리본부, "Public health Weekly Report 2(3)" 697-702, 2009

26 질병관리본부, "Public health Weekly Report 2(2)" 681-686, 2009

27 Clinical and Laboratory StandardsInstitute, "Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals.2nd ed"

28 질병관리본부, "PFGE(Pulsed Field Gel Electrophoresis)표준실험법"

29 Mitarai Y, "Outbreaks of Salmonella Enteritidis infection in broiler chickens" 31 : 92-99, 1995

30 CDC (Pulsenet), "One-Day(24-28 h)Standardized Laboratory Protocol for Molecular Subtyping of Escherichia coli O157:H7,non-typhoidal Salmonella serotypes,and Shigella sonnei by Pulsed Field Gel Electrophoresis(PFGE)"

31 NARMS, "National Antimicrobial Resistance Monitoring System-Enteric Bacteria"

32 Gast RK, "Isolation of Salmonella Enteritidis from internal organs of experimentally infected hens" 34 : 991-993, 1990

33 Rodrigue DC, "International increase in Salmonella Enteritidis: A new pandemic?" 105 : 21-27, 1990

34 Tenover FC, "How to select and interpret molecular strain typing methods for epidemiological studies of bacteria infections: a review for healthcare epidemiologists. Molecular Typing Working Group of the Society for Healthcare Epidemiology of America" 18 (18): 426-439, 1997

35 FDA/CFSAN, "Food Safety A to Z Reference Guide-Salmonella"

36 Ewing WH, "Edward and Ewing’s Identification of Enterobacteriaceae" Elsevier Science Publishing Co 181-318, 1986

37 David LH, "Control of Communicable Diseases Manual.18th ed"

38 FDA, "Center for drug evaluation and research"

39 Grimont P.A.D, "ANTIGENIC FORMULAE OF THE SALMONELLA SEROVARS" Institut Pasteur 2007