DNA barcode of the uncertain species of the family Tortricidae (Lepidoptera), Archips audax Razowski and A. asiaticus Walsingham

다국어 초록 (Multilingual Abstract)

This study was carried out to clarify the taxonomic status position between two species, Archips audax and A. asiaticus of the family Tortricidae, which have been confused due in their very similar character in appearance. For the reason, A. asiaticus has been treated as junior of A. audax in Korea until now (Park and Byun, 1989). Also, it has been known as synonym of A. audax too in Japan (Suzuki and Komai, 1984). However, in China A. asiaticus has been listed as a valid species (Park et al,. 2014). The aim of this study is to clarify the taxonomic differences between the two species with DNA barcoding. We conducted the tree with the result of DNA barcoding by using MAGA 6. A. audax and A. asiaticus build in different clade and distance was 0.02. In this study, we extracted COI gene for DNA barcode and analyzed the sequences using MEGA 6. Also, genitalic dissection and observation of morphology were conducted. As the result, we have to treated A. asiaticus is valid species.