RAPD 마커를 이용한 한국밀의 유전적 다양성 평가 및 품종 판별

다국어 초록 (Multilingual Abstract)

Eleven RAPD primers were assessed to analyze genetic diversity of Korean wheat varieties and to develop DNA marker for cultivar identification. The average of the number of polymorphic bands was 5.2 and PIC values showed 0.48, respectively. Ten major clades were presented by phylogenetic analysis. Three cultivars containing Uri, Hanbeak and Jonong were distinct from the others in the phylogenetic dendrogram. Seven cultivar-specific fragments were detected from 11 RAPD fingerprinting among 35 wheat cultivars and they were sequenced. Four Korean wheat cultivars, Eunpa, Jopoom, Yeonbaek and Jeokjoong, were identified newly by four markers, 84, 173, 174 and KWSM011. We convince that these new DNA markers are useful for cultivar fingerprinting and are applied to marker-assisted selection in wheat breeding program.

목차 (Table of Contents)

• 서 언
• 재료 및 방법
• Genomic DNA 추출
• RAPD 다형성 분석
• 클로닝 및 염기서열 분석
• 유전적 유연관계 분석
• 분자마커 제작 및 품종 적용
• 결과 및 고찰
• RAPD 다형성 분석
• 유전적 유연관계 분석
• 품종 특이적 프라이머 디자인 및 적용
• 적 요
• 사 사
• REFERENCES

참고문헌 (Reference)

1 Gupta PK, "Wheat genomics: present status and future prospects" 896451-, 2008

2 Liu BH., "Statistical genomics: linkage, mapping and QTL analysis" CRC Press 1998

3 Wang RRC, "Production and identification of chromosome specific RAPD markers for durum wheat ‘Langdon’ disomic substitution lines" 35 : 886-888, 1995

4 Landry BS, "Phylogeny analysis of 25 apple rootstocks using RAPD markers and tactical gene tagging" 89 : 847-852, 1994

5 Nesbitt KA, "Partioning and distribution of RAPD variation in a forest tree species, Eucalyptus globules (Myrtaceae)" 74 : 628-637, 1995

6 Rohlf FJ., "NTSYS-PC. Numerical taxonomy and multivariate analysis system, version 2.00"

7 Pakniyat H, "Markers associated with drought tolerance in bread wheat (Triticum aestivum L.)" 10 : 3237-3239, 2007

8 손재한, "ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification" 한국육종학회 1 (1): 262-269, 2013

9 Rafalski JA, "Genetic diagnostics in plant breeding: RAPDs, Microsatellites and Machines" 9 : 275-280, 1993

10 Kuchel H, "Genetic and economic analysis of a targeted marker-assisted wheat breeding strategy" 16 : 67-78, 2005

11 De Freitas LB, "Extreme homogeneity among Brazilian wheat genotypes determined by RAPD markers" 35 : 2255-2260, 2000

12 한손재, "Development of SCAR Markers for Korean Wheat Cultivars Identification" 한국육종학회 2 (2): 224-230, 2014

13 Chen JM, "Development and characterization of Elymus rectisetus species and accession-specific RAPD markers" Univ. Extension Press. Univ. of Saskatchewan 3 : 98-101, 1998

14 Chalmers KJ, "Detection of genetic variation between and within populations of Gliricidia sepium and G. maculate using RAPD markers" 69 : 465-472, 1992

15 He S, "Detection of DNA sequence polymorphisms among wheat varieties" 84 : 573-578, 1992

16 Wiliams JGK, "DNA polymorphisms amplified by random primers are useful as genetic markers" 18 : 6531-6535, 1990

17 Dweikat I, "Association of a DNA marker with the hessian fly resistance gene H9 in wheat" 89 : 964-968, 1994

18 Grewal S, "Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers" 6 : 18-23, 2007

19 박철수, "Allelic Variation of Glutenin, Granule-Bound Starch Synthase I and Puroindoline in Korean Wheat Cultivar" 한국작물학회 54 (54): 181-191, 2009