별불가사리(Asteriaa pectinifera) 렉틴의 사이토카인 생성 양상 = Effect of Asterina pectinifera Lectin on Cytokine Production

다국어 초록 (Multilingual Abstract)

The purpose of this study is to define whether Asterina pectinifera Lectin (APL) is effective on the cytokine production. Isolated mRNA from hPBMC (human peripheral blood mononuclear cells) stimulated with APL for various reaction times (1 to 96 hours) was detected by RT-PCR. The intensity of band for IL-1 and IFNα mRNA was markedly increased at 1 hour, and IL-2 mRNA was strongly expressedat 4 hours. The mRNA band of APL-induced IL-2 and TFNα was weaker than that of IL-1, IL-6 and TNFα. The mRNA expression of 4 cytokines (IL-1, IL-2, IFN?? and TNFα) was detected up to 48 hours, and that of IL-6 was detected until 72 hours. ELISA was used to look protein secretion of the cytokine gene with IL-1, IL-2 and TNFα expressed strongly in RT-PCR. The highest protein secretion was at 4 hours with IL-1, at 8 hours with IL-2 and at 4 hours with TNFα. These results suggest that APL can induce the pro-duction of some cytokines and the immune response from PBMC was done within the first few hours of stimulation with APL.