Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis

다국어 초록 (Multilingual Abstract)

Purpose: At present, commercially available antiurolithic drugs have more adverse effects than potential therapeutic or preventive effects with chronic use. With this in mind, the present study was designed to assess the antiurolithic effect of olive oil in a mouse model of ethylene glycol (EG)-induced urolithiasis.
Materials and Methods: Adult albino mice were divided into 6 groups. Group I was fed the vehicle only. Group II was supplemented with 0.75% EG alone in drinking water during the experimental period to initiate deposition of calcium oxalate in kidneys, which leads to urolithiasis in animals. Groups III (olive oil control group) through V were fed olive oil orally at various doses during the experimental period. Group VI received cystone (750 mg/kg). Groups IV–VI additionally received 0.75% EG in drinking water ad libitum. SPSS ver.17.0 was used for statistical analysis.
Results: The study results showed significantly higher levels of serum urea, uric acid, and creatinine (p<0.05) in group II than in groups III–VI and I. Administration of olive oil at different doses restored the elevated serum parameters in groups IV and V compared with group II. Urine and kidney calcium, oxalate, and phosphate levels in groups IV–VI were significantly lower (p<0.05) than in animals with EG-induced urolithiasis (group II). Group V mice showed a significant restoration effect on serum as well as urine and kidney parameters compared with group II.
Conclusions: Supplementation with olive oil (1.7 mL/kg body weight) reduced and prevented the growth of urinary stones, possibly by inhibiting renal tubular membrane damage due to peroxidative stress induced by hyperoxaluria.

참고문헌 (Reference)

1 Fossati P, "Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine" 26 : 227-231, 1980

2 Basavaraj DR, "The role of urinary kidney stone inhibitors and promoters in the pathogenesis of calcium containing renal stones" 5 : 126-136, 2007

3 Robertson WG, "The cause of idiopathic calcium stone disease: hypercalciuria or hyperoxaluria?" 26 : 105-110, 1980

4 Hu FB, "The Mediterranean diet and mortality--olive oil and beyond" 348 : 2595-2596, 2003

5 Christina AJ, "Studies on the antilithic effect of Rotula aquatica lour in male Wistar rats" 24 : 357-359, 2002

6 Kishimoto T, "Side effects of extracorporeal shock-wave exposure in patients treated by extracorporeal shock-wave lithotripsy for upper urinary tract stone" 12 : 308-313, 1986

7 Tripathi KD, "Relevant physiology of urine formation; essentials of medical pharmacology." 77 : 557-560, 2005

8 Soldati L, "Relevance of Mediterranean diet and glucose metabolism for nephrolithiasis in obese subjects" 12 : 34-, 2014

9 O. Benavente-Garcia, "Radioprotective Effects In Vivo of Phenolics Extracted from Olea europaea L. Leaves Against X-Ray-Induced Chromosomal Damage:" 한국식품영양과학회 5 (5): 125-135, 2002

10 Atmani F, "Prophylaxis of calcium oxalate stones by Herniaria hirsuta on experimentally induced nephrolithiasis in rats" 92 : 137-140, 2003

11 Coe FL, "Plaque and deposits in nine human stone diseases" 38 : 239-247, 2010

12 Miller NL, "Pathogenesis of renal calculi" 34 : 295-313, 2007

13 Poudyal H, "Olive leaf extract attenuates cardiac, hepatic, and metabolic changes in high carbohydrate-, high fat-fed rats" 140 : 946-953, 2010

14 OECD, "OECD guidelines for acute toxicity of chemicals: acute oral toxicity – fixed dose procedure" Paris (France): Organisation for Economic Co-operation and Development 2001

15 Grases F, "New aspects on the composition, structure and origin of calcium oxalate monohydrate calculi" 24 : 381-386, 1993

16 Ghodkar PB, "In: Godkar PB, Godkar DP. Textbook of medical laboratory technology" Mumbai:Bhalani Publishing House 118-132, 1994

17 Komaki, "Identification of anti-alpha-amylase components from olive leaf extracts" 9 : 35-39, 2003

18 Lemann J Jr, "Hypercalciuria and stones" 17 : 386-391, 1991

19 Fan J, "Examination of crystalluria in freshly voided urines of recurrent calcium stone formers and normal individuals using a new filter technique" 161 : 1685-1688, 1999

20 Asplin J, "Evidence that nephrocalcin and urine inhibit nucleation of calcium oxalate monohydrate crystals" 261 (261): F824-F830, 1991

21 Trinchieri A, "Epidemiological trends in urolithiasis: impact on our health care systems" 34 : 151-156, 2006

22 Afonne OJ, "Effects of rinbacin extract on rat kidney" 25 : 1022-1025, 2002

23 Mitra SK, "Effect of cystone, a herbal formulation, on glycolic acidinduced urolithiasis in rats" 12 : 372-374, 1998

24 Flynn MM, "Comparing an olive oil-enriched diet to a standard lower-fat diet for weight loss in breast cancer survivors:a pilot study" 19 : 1155-1161, 2010

25 Cheesman EE, "Classification of the bananas. the genus Ensete Horan" 2 : 97-106, 1968

26 Somova LI, "Cardiotonic and antidysrhythmic effects of oleanolic and ursolic acids, methyl maslinate and uvaol" 11 : 121-129, 2004

27 Khayyal MT, "Blood pressure lowering effect of an olive leaf extract (Olea europaea) in L-NAME induced hypertension in rats" 52 : 797-802, 2002

28 Bashir S, "Antiurolithic effect of Bergenia ligulata rhizome: an explanation of the underlying mechanisms" 122 : 106-116, 2009

29 Nayeem K, "Antiurolithiatic potential of the fruit extracts of Carica papaya on ethylene glycol induced urolithiatic rats" 3 : 2772-2775, 2010

30 Christina AJ, "Antilithiatic effect of Melia azedarach. on ethylene glycol-induced nephrolithiasis in rats" 44 : 480-485, 2006