대식세포와 암에서의 에피신 발현에 대한 연구

다국어 초록 (Multilingual Abstract)

Epithin is a TypeII Serine Protease. It functions in cell adhesion & migration, and development of epithelial cell in the embryo. Also it's overexpression was found during tumorogenesis. In the earlier study, epithin was found to be expressed in macrophage, when activated with IFN-gamma. In this study with signal pathway inhibitors, JAK signal pathway in RAW264.7 macrophage cell line and bone marrow derived macrophage induces expression of epithin. In addition, 4T1 breast cancer mouse model was established to study the expression of epithin in tumor associated macrophage. CD11b+ cells were detected in the outside of cancer in early stage of tumor. Over time, it was located in cancer. In the all of tumor progression stages, Fractions of CD11b+ cells expressed epithin in the tumor microenvironment. These results suggest potential functions of epithin in macrophages in inflammatory and tumor microenvironments.

목차 (Table of Contents)

• Index 4
• Abstract 6
• Introduction 7
• Materials and Methods 9
• 1. Mice and Cells 9
• 2. Reagents 9
• 3. Cytokine and inhibitors treatment 9
• 4. Western blot 10
• 5. Bone marrow derived macrophage 10
• 6. Flow cytometry 11
• 7. Breast cancer mouse model 11
• 8. Live imaging system 12
• 9. Immunohistochemistry 12
• Result 14
• 1. Epithin is induced in macrophage cell line by IFN- through JAK pathway 14
• 2. IFN-/JAK pathway induces expression of epithin in primary macrophage 15
• 3. Character of breast cancer mouse model 16
• 4. 4T1tdTomato breast cancer cells were detected by live imaging system. 16
• 5. Expression pattern of epithin in macrophages are shown in breast cancer mouse model 17
• Discussion 19
• Figures 20
• Reference 33