Study on the Separation of fetal nucleated red blood cells for non-invasive prenatal diagnosis = 비침습적 산전진단을 위한 태아 유핵 적혈구 분리

목차 (Table of Contents)

• Abstract .......................................................................................................... ⅰ
• List of Tables .................................................................................................. ⅴ
• List of Figures ................................................................................................ ⅴ
• 1. Introduction ................................................................................................. 1
• 2. Materials and methods ............................................................................... 3
• 2.1 Working principle .................................................................................... 3
• 2.2 Design ...................................................................................................... 6
• 2.3 Fabrication process of the lateral magnetophoretic microseparator ........ 8
• 2.4 Blood collection..................................................................................... 11
• 2.5 RBC hyperaggregation process ............................................................. 11
• 2.6 Fluorescence-activated cell sorting ....................................................... 11
• 2.7 Sample preparation for the lateral magnetophoretic microseparator ..... 12
• 2.8 Immunofluorescence staining ................................................................ 12
• 2.9 Whole genome amplification and PCR ................................................. 13
• 3. Results and discussion .............................................................................. 14
• 3.1 Positive enrichment process .................................................................. 14
• 3.2 Negative enrichment process ................................................................. 20
• 3.3 Isolation of NRBCs in maternal blood .................................................. 22
• 4. Conclusions ................................................................................................ 28
• 5. References .................................................................................................. 29
• Appendix : Fabrication Runsheet ................................................................ 31