생쥐에서 키토산의 조직학적·세포유전학적 안전성 및 방사성수은(203HgCl₂) 흡착 효과 = Histological and cytogenetic safety of chitosan and its chelation effects on radiomercury(203HgCl₂) in mice

다국어 초록 (Multilingual Abstract)

The purpose of this study was to evaluate the histological and cytogenetic toxicity of chitosan that was a deacetylated derivative of chitin. Also, the effects of chitosan on the excretion of radiomercury (^(203)HgCl_(2)) and suppression of chromosome aberration caused by radiomercury were determined.
The frequency of micronuclei occurrence was observed after administrating 0.01, 0.1, and 1% chitosanoligosaccharide for 7, 60, and 180 days ad libitum. The frequency of the micronucleus Occurrence not associated with the intake periods and concentrations (p>0.05).
After administrating the frequency of chromosomal aberration was observed after administrating 0.01, 0.1, and 1% chitosan oligosaccharide to mice, in F_(1), F_(2), F_(3) generations and adults. The frequency of the chromosomal aberration Occurrence was not associated with the intake periods and concentrations (P>0.05).
After treated with the 0.1 % (1 mg/㎖) chitosanoligosaccharide was supplied for 90, 365 days ad libitum The blood components did not changed (P>0.05).
For the evaluation of chitosan safety, the ultrastructural changes of the liver in F_(1), F_(2), F_(3) generations and adults were observed with an electromicroscope (EM) after administrating 0.1% (1 mg/㎖) chitosanoligosaccharide to rats ad libitum. In case of F_(2) and 90-day treatment gropus, the endoplasmic reticulum (ER) was extended and ribosomes dropped from the ER. Other groups did not changed under the EM.
The chelation capacity of the radiomercry between non-phos phorylated chitosan and phosphorylated chitosan was evaluated according to the times, degree of deacetylation (DAC), pH and phosphorus contents. The phosphorylated chitosan showed higher chelation efficiency on radiomercury than that of non-phophory lated chitosan (P<0.0l).
In spite of different times, DAC and pH, the phophorylated chito san was no specific differences in absorption efficiency with DAC and phosphorus content (P>0.05). And, the non-phosphorylated chitosan was higher chelation efficiency on radiomercury according to increased DAC and times (P<0.01)
After contaminated radiomercury (0.005 μCi/b.w(g)) through oral, intraperitoneal and intravenous injection, the phophorylated chitosan and chitosanoligosaccharide was fed through the same route as above. Only the feces of the oral contaminated was effective (P<0.01)
For the observation of suppression effect on radiomercury transfer through milk, the mother mice were orally contaminated with radiomercury (0.005 μCi/b.w(g)), and then 1% chitosanoligo saccharide was orally injected. The chitosanoligosaccharide significantly reduced the milk transfer of radiomercury (P<0.05).
For the observation of suppression effect on the chromosomal aberration, 0.1, 0.5, 1% chitosanoligosaccharide was orally pretreated for 30 days ad libitum. And, mice were orally contaminated with 0.005 μCi/b.w(g) radiomercury. The chitosan oligosaccharide significantly reduced the chromosomal aberration caused by radiomercury (P<0.05).
It was concluded that the chitosanoligosaccharide was a nontoxic material in parts of histological and cytogenetic safety. And, the chitosanoligosaccharide effectively excreted radiomercury by feces.

목차 (Table of Contents)

• 목차
• Ⅰ. 서론 = 1
• Ⅱ. 재료 및 방법 = 6
• 1) 실험에 사용한 키토산올리고당의 분자량 및 점도 확인 = 6
• 2) 생쥐에서 키토산올리고당 섭취에 따른 미소핵 검사 (micronuclei test) = 7
• 3) 생쥐에서 키토산올리고당 섭취에 따른 염색체이상 검사 (chromosomal aberration test) = 10
• 4) 키토산올리고당을 흡수한 생쥐에서 혈액학적 성분의 변화 = 13
• 5) 키토산올리고당을 섭취한 쥐에서 각 세대별 간(liver) 미세조직의 구조 변화 = 15
• 6) 키토산인산화에 시간별, pH별 방사성수은 흡착 효과 (in vitro test) = 17
• 7) 방사성수은에 오염된 생쥐에서 각 투여 경로별 인산화 키토산, 키토산올리고당에 의한 방사성수은의 변(feces)을 통한 체외 배출 촉진 효과 = 20
• 8) 방사성수은에 오염된 어미생쥐 모유를 통한 어린 새끼로의 전이 억제에 관한 키토산의 효과 = 22
• 9) 생쥐에서 방사성수은 오염에 의한 염색체이상에 관한 키토산올리고당의 억제 효과 = 24
• Ⅲ. 결과 = 26
• 1) 실험에 사용한 키토산올리고당의 분자량 및 점도 결과 = 26
• 2) 생쥐에서 키토산올리고당 섭취에 따른 미소핵 검사 = 28
• 3) 생쥐에서 키토산올리고당 섭취에 따른 염색체이상 검사 = 31
• 4) 키토산올리고당을 섭취한 생쥐에서 혈액학적 성분의 변화 = 36
• 5) 키토산올리고당을 섭취한 쥐에서 각 세대별 간 미세조직의 구조 변화 = 40
• 6) 인산화키토산에 따른 시간별, pH별 방사성수은 흡착효과 (in vitro) = 44
• 7) 방사성수은에 오염된 생쥐에서 각 투여 경로별 인산화 키토산, 키토산올리고당에 의한 방사성수은의 변을 통한 체외 배출 촉진 효과 = 46
• 8) 방사성수은에 오염된 어미생쥐의 모유를 통한 어린 새끼로의 전이 억제에 관한 키토산의 효과 = 53
• 9) 생쥐에서 방사성수은 오염에 의한 염색체이상에 관한 키토산올리고당의 억제 효과 = 56
• Ⅳ. 고찰 = 63
• Ⅴ. 결론 = 73
• Ⅵ. 참고문헌 = 75