황색포도상구균 분리 배지의 검출효율 및 정확도 비교

다국어 초록 (Multilingual Abstract)

The purpose of this study was to evaluate the detection efficiency and accuracy of Baird parker agar(BPA) and MC-Media Pad SA(MMPSA) with
Staphylococcus aureus reference strains, wild strains and monitoring food. As
a result of comparing the specificity of BPA and MMPSA, S. aureus reference strain and wild strains produced typical colonies on BPA and MMPSA, but non-S. aureus showed atypical colonies or did not grow on BPA and MMPSA. The recovery of reference strain from artificially inoculated foods was 2.8 ± 1.2 log CFU/g in BPA and 2.9 ± 1.1 log CFU/g in MMPSA. The correlation coefficient(R2) of recovery rates between BPA and MMPSA was 0.986. The detection efficiency of monitoring foods was 53.3% in BPA and MMPSA. The quantitative accuracy of food monitoring were 2.0 ± 0.8 log CFU/g in BPA and 2.0 ± 0.7 log CFU/g in MMPSA.
The correlation coefficient(R2) of quantitative accuracy was 0.980. The qualitative accuracy of food monitoring were 97.7% in BPA and 96.4% in MMPSA. The detection efficiency, qualitative and quantitative accuracy for selective medium were not significantly different, so it was judged that MMPSA, along with BPA, could be used for isolation, enumeration and identification of S. aureus in various foods.

목차 (Table of Contents)

• Ⅰ. 서 론 1
• Ⅱ. 재료 및 방법 4
• 1. 실험 재료 4
• 2. 실험 방법 7
• Ⅲ. 결과 및 고찰 11
• 1. 표준 균주를 이용한 황색포도상구균 분리 배지의 특이도 비교 11
• 2. 표준 균주를 이용한 황색포도상구균 분리 배지의 회수율 비교 15
• 3. 모니터링을 통한 황색포도상구균 분리 배지의 검출효율 및 정량 정확도 비교 34
• 4. 모니터링을 통한 황색포도상구균 분리 배지의 정성 정확도 비교 40
• Ⅳ. 요 약 44
• Ⅴ. 참고문헌 46