Water uptake through roots, is an essential function of the water circulation of plants, and it is indispensable for understanding the whole sap flow dynamics. In this study, the tips of Arabidopsis’ root hairs were excised and exposed to dehydratio...
Water uptake through roots, is an essential function of the water circulation of plants, and it is indispensable for understanding the whole sap flow dynamics. In this study, the tips of Arabidopsis’ root hairs were excised and exposed to dehydration and rehydration cycles. During this process, the water-refilling process inside the xylem vessels of root hairs was visualized using the synchrotron X-ray imaging technique which has a high spatial resolution of 2 ㎛. High temporal resolution and sufficient beam intensity of the X-ray source make it possible to record consecutive images of the water-refilling process up to 10 frames/sec. Various flow patterns were observed and the ascending speed of the water-air interfaces was analyzed. The relations between the upward-rising height and ascending speed were also analyzed. The present results would be helpful for understanding sap flows in roots.