家兎 肝의 Adenosine Diphosphate Ribose Pyrophosphohydrolase의 性狀 = Adenosine Diphosphate Ribose Pyrophosphohydrolase of the Rabbit Liver

다국어 초록 (Multilingual Abstract)

Adenosine diphosphate ribose pyrophosphohydrolase(ADPRase), which catalyzes the hydrolysis of ADPR to yield AMP and ribose-5'-phosphate was widely distributed in the cytoplasm of rabbit tissues. All the activity of ADPRase found in the cytoplasm of the erythrocytes was Mg^2+dependent and that of the kindey or the pancreas was Mg^2+independent, while cytoplasmic ADPRases of the liver consist of both the Mg^2+independent(92%) and Mg^2+independent(8%) form. Shown by the subcellular distributon of the Mg^2+independent ADPRase in the liver, 65% of the total activity was found in the nucleus, 20% in mitochondria, and 15% in cytoplasm.
Cytoplasmic or nucleal Mg^2+independent ADPRase of the kidney was specifically inhibited by AMP but not by ATP at all, while both forms of ADPRase of the liver were inhibited not only by AMP but by ATP to the larger extent.
It is proposed that Mg^2+independent ADPRase would convert to Mg^2+independent form by the storage of this enzyme at 4℃, showing that the activity of the dependent form became the same as independent form if stored a day and further increasement of independent form attained to the 85% of the total activity in five days storage.