Optimizing the composition of the medium for the viable cells of Bifidobacterium animalis subsp. lactis JNU306 using response surface methodology = Optimizing the composition of the medium for the viable cells of Bifidobacterium animalis subsp. lactis JNU306 using response surface methodology

다국어 초록 (Multilingual Abstract)

This research improved the growth potential of Bifidobacterium animalis subsp lactis strain JNU306, a commercial medium that is appropriate for large-scale production, in yeast extract, soy peptone, glucose, L-cysteine, and ferrous sulfate. Response surface methodology (RSM) was used to optimize the components of this medium, using a central composite design and subsequent analyses. A second-order polynomial regression model, which was fitted to the data at first, significantly lacked fitness. Thus, through further analyses, the model with linear and quadratic terms plus two-way, three-way, and four-way interactions was selected as the final model. Through this model, the optimized medium composition was found as 2.8791% yeast extract, 2.8030% peptone soy, 0.6196% glucose, 0.2823% L-cysteine, and 0.0055% ferrous sulfate, w/v. This optimized medium ensured that the maximum biomass was no lower than the biomass from the commonly used blood-liver (BL) medium. The application of RSM improved the biomass production of this strain in a more cost-effective way by creating an optimum medium. This result shows that B. animalis subsp lactis JNU306 may be used as a commercial starter culture in manufacturing probiotics, including dairy products.

참고문헌 (Reference)

1 Yuichi OCHI, "STUDIES ON LACTOBACILLI" Japanese Society of Veterinary Science 20 (20): 71-81_3, 1958

2 SAS Institute, "SAS/STAT user’s guide, release 6.04"

3 Kantha D. Arunachalam, "Role of Bifidobacteria in nutrition, medicine and technology" Elsevier BV 19 (19): 1559-1597, 1999

4 Marcos Almeida Bezerra, "Response surface methodology (RSM) as a tool for optimization in analytical chemistry" Elsevier BV 76 (76): 965-977, 2008

5 André I. Khuri, "Response surface methodology" Wiley 2 (2): 128-149, 2010

6 S Oh, "Optimizing conditions for the growth of Lactobacillus casei YIT 9018 in tryptone-yeast extract-glucose medium by using response surface methodology" American Society for Microbiology 61 (61): 3809-3814, 1995

7 S.L. Liew, "Optimization of medium composition for the production of a probiotic microorganism, Lactobacillus rhamnosus, using response surface methodology" Elsevier BV 102 (102): 137-142, 2005

8 Kristiina Kiviharju, "Optimization of a Bifidobacterium longum production process" Elsevier BV 117 (117): 299-308, 2005

9 Sen R, "Modeling and optimization of the process conditions for biomass production and sporulation of a probiotic culture" 40 : 2531-2538, 2007

10 A. M. Yazid, "Growth Optimization of a Probiotic Candidate, Bifidobacterium pseudocatenulatum G4, in Milk Medium Using Response Surface Methodology" 한국생물공학회 12 (12): 106-113, 2007

11 R.I. Dave, "Evaluation of Media for Selective Enumeration of Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus, and Bifidobacteria" American Dairy Science Association 79 (79): 1529-1536, 1996

12 Montgomery DC, "Design and analysis of experiments" John Wiley & Sons 1984

13 L. Masco, "Culture-dependent and culture-independent qualitative analysis of probiotic products claimed to contain bifidobacteria" Elsevier BV 102 (102): 221-230, 2005

14 Rodolphe Barrangou, "Comparison of the complete genome sequences of Bifidobacterium animalis subsp" American Society for Microbiology 191 (191): 4144-4151, 2009

15 Hussain SM, "Bioprocess development for enhanced production of probiotic Bifidobacterium bifidum" 118 : 280-285, 2020

16 Bezcorovainy A, "Biochemistry and physiology of bifidobacteria" CRC press 1989

17 Teemu Taipale, "Bifidobacteriumanimalis subsp. lactis BB-12 in reducing the risk of infections in infancy" Cambridge University Press (CUP) 105 (105): 409-416, 2011

18 Marco Ventura, "Bifidobacterium lactis DSM 10140: identification of the atp (atpBEFHAGDC) operon and analysis of its genetic structure, characteristics, and phylogeny" American Society for Microbiology 70 (70): 3110-3121, 2004

19 Rašić J, "Bifidobacteria and their role: microbiological, nutritional-physiological, medical and technological aspects and bibliography" Birkhäuser Verlag 1983

20 Borja Sánchez, "Adaptation and response of Bifidobacterium animalis subsp. lactis to bile: a proteomic and physiological approach" American Society for Microbiology 73 (73): 6757-6767, 2007

21 M. R. W. Brown, "A simple diagnostic milk medium for Pseudomonas aeruginosa" BMJ 23 (23): 172-177, 1970