로즈마리와 α-Tocopherol Acetate의 급여가 육계의 생산성 및 냉장 저장 중 계육의 품질에 미치는 영향 = Effects of Dietary Supplementation with Rosemary and α-Tocopherol Acetate on Performance and Meat Quality of Chicken Meat during Refrigerated Storage

다국어 초록 (Multilingual Abstract)

The effects of rosemary and α-tocopherol, added individually or in combination, on broiler performance, thiobarbituric acid reactive substance (TBARS), total plate count (TPC) and meat color of chicken thigh meat were investigated. Three hundred broiler chicks divided into five groups were fed a basal diet (control) or basal diet supplemented with 5 g rosemary/kg (T1), 10 g rosemary/kg (T2), 200 mg α-tocopherol/kg (T3), or 5 g rosemary/kg + 200 mg α-tocopherol/kg (T4) for 5weeks. Following slaughter, chicken meat was stored at 4oC for 10 days. All treatments did not influence the performance.
Rosemary supplementation delayed lipid oxidation in thigh meat during refrigerated storage. T2 was significantly (p<0.05)more effective in delayed lipid oxidation compared to T1, but was inferior to T3. Samples containing a combination of antioxidant had lower TBARS values than those containing the individual antioxidants, indicating a synergistic effect. TPC was significantly increased (p<0.05) in thigh meat of all groups throughout the refrigerated storage. The T3 and control groups showed TPC counts that did not differ from each other during the entire storage period. However, rosemary supplementation was associated with bacterial counts that were significantly lower (p<0.05) than the control and α-tocopherol groups at day 3 of storage and thereafter. For this period, T1 presented TPC counts that were significantly higher than the T2 group (p<0.05). At all storage times, the thigh meat of rosemary-fed chickens was redder than control (higher a*), while no differences in L* and b* values were found. A synergistic effect was obtained from the combination of rosemary with α-tocopherol,whereas individual use of the antioxidants significantly improved color stability compared to the control.

국문 초록 (Abstract)

본 시험은 육계 300수를 로즈마리와 α-tocopherol acetate를 급여하여 5주간 사육한 육계의 생산성과 계육의 TBARS,총미생물수, pH, 및 육색을 조사하였다. 육질 분석은 계육을 냉장 보관하면서 실험하였다. 시험구는 무첨가구를 대조구(Control)로 하고, 로즈마리 5 g/kg 급여구를 T1, 로즈마리 10 g/kg 급여구를 T2, α-tocopherol acetate 200 mg/kg 급여구를 T3, 그리고 로즈마리 5 g/kg과 α-tocopherol acetate 200 mg/kg 급여구를 T4 등 5개 처리구로 나누어사양하였다. 육계의 증체량, 사료섭취량 및 사료요구율 등의 생산성은 유의성이 없었다. TBARS는 모든 처리구에서 저장기간이 경과하면서 증가하였고, T2, T3 및 T4에서대조구와 T1보다 유의하게 낮아 로즈마리와 α-tocopherol은 저장성을 향상시키는 결과이었다. 로즈마리 급여량이많은 T2가 T1보다, 로즈마리와 α-tocopherol 혼합 급여구에서 TBARS는 낮았다. pH는 저장기간이 지남에 따라 감소하였고, 처리구간의 유의성은 없었다. 총미생물수는 저장기간이 지나면서 모든 처리구에서 증가하였고, α-tocopherol은 미생물의 감소 효과는 없으나 로즈마리와 로즈마리, α-tocopherol 혼합 급여는 대조구와 T3보다 유의하게 낮았다(p<0.05). 육색은 L*, a*, 및 b* 값은 저장하면서 모두 감소하였고, 로즈마리와 로즈마리, α-tocopherol 혼합 급여구에서 a*값이 유의하게 높았다(p<0.05). 로즈마리 및 로즈마리와 α-tocopherol의 급여구에서 항산화 및 육색의 산화 안정성이 향상되었고, 혼합 급여구에서 시너지 효과가 있었다.

참고문헌 (Reference)

1 Buttris, J. A., "The α-tocopherol and phospholipids fatty acid content of rat liver subcellula membrances in vitamin E and selenium deficiency" 963 : 611-615, 1988

2 Govaris, A., "The inhibitory potential of feed supplementation with rosemary and/ or α- tocopherol acetate on microbial growth and lipid oxidation ofturkey breast during refrigerated storage" 40 : 331-337, 2007

3 O’Sullivan, M. G., "The effect of dietary vitamin E supplementation on quality aspects of porcine muscle" 37 : 227-235, 1998

4 Brewer, M. S., "TBA values, sensory characteristics and volatiles in ground pork during long-term frozen storage: Effect of packing" 57 : 558-564, 1992

5 Dirinck, P., "Studies on vitamin E and meat quality. 1. Effect of feeding high vitamin E levels on time relatived pork quality" 44 : 65-70, 1996

6 Ito, N., "Studies on antioxidants: their carcinogenic and modifying effects on chemical carcinogenesis" 24 : 1071-1082, 1986

7 Rice-Evans, C. A., "Structure and phenolic acids" 20 : 933-956, 1996

8 Holley, R. A., "Static, controlled (CO2) atmosphere packing of retail ready pork" 59 : 1296-1301, 1994

9 Williams, G. M., "Safety assessment of hydroxyanisole and butylated hydroxytoluene as antioxidant food additives" 37 : 1027-1038, 1999

10 SAS Institute Inc, "SAS/STAT User`s Guide: Version 8.2. SAS Institute, Inc., Cary, NC"

11 Perez-Fons, L., "Rosemary diterpense affect lipid polymorphism and fluidity in phospholipids membranes" 453 : 224-236, 2008

12 Thorsen, M. A., "Quantitative determination of phenolic diterpenes in rosemary extracts. Aspect of accurate quantification" 995 : 119-125, 2003

13 Sofos, J. N., "Potential interaction between antioxidant and microbial meat quality. in: Antioxidant in muscle foods" Willey 427-453, 2000

14 Shahidi, F., "Natural phenolic antioxidants and their food applications" 12 : 80-84, 2000

15 Frankel, E. N., "Lipid oxidation, mechanism, products and biological significance" 61 : 1908-1914, 1984

16 Lanari, M. C., "Kinetic of pigment oxidation in beef from steers supplemented with vitamin E" 61 : 884-889, 1996

17 Schwarz, K., "Investigation of plant extracts for the protection of processed foods against lipid oxidation. Comparison of antioxidant assays based on radical scavenging, lipid oxidation and analysis of the principal antioxidant compounds" 212 : 319-328, 2001

18 Hernandez, F., "Influence of two plant extracts on broiler performance, digestibility, and digestive organ size" 83 : 169-174, 2004

19 Monahan, F. J., "Influence of dietary vitamin E (α- tocopherol) on the colour stability of pork chops" 543-546, 1992

20 Machlin, L. J., "In vitamin E. A comprehensive treatise. in: Basic and clinical nutrition. 1" Marcel Dekker, Inc. 1980

21 Cannon, J. E., "Growth and fresh meat quality characteristics of pigs supplemented with vitamin E" 74 : 98-103, 1996

22 Camo, J., "Extention of the display life of lamb with an antioxidant active packing" 80 : 1086-1091, 2008

23 McCarthy, T. L., "Evaluation of the antioxidant potential of natural food/plant extracts as compared with synthesis antioxidant and vitamin E in raw and cooked pork patties" 57 : 45-52, 2001

24 Singh, N., "Efficicacy of choline, dioxide, ozone and thyme essential oil or a sequential washing in killing E. coli O157: H7 on lettuce and baby carrots" 35 : 720-729, 2002

25 Ahn, J. H., "Effects of plant extract on microbial growth color change, and lipid oxidation in cooked beef" 24 : 7-14, 2007

26 Lin, C. F., "Effects of dietary oils and α- tocopherol supplementation on lipid composition and stability of broiler meat" 54 : 1457-1460, 1989

27 Asghar, A., "Effect of supranutritional dietary vitamin E levels on subcellar deposition of α-tocopherol in the muscle and pork quality" 57 : 31-41, 1991

28 Haak, L., "Effect of dietary rosemary and a-tocoperyl acetate on the oxidative stability of raw and cooked pork following oxidized linseed oil administration" 78 : 239-247, 2008

29 Sherbeck, J. A., "Dietary supplementation of vitamin E to feedlot cattle affects beef retail display properties" 60 : 250-252, 1995

30 Chen, S. C., "Chemical discrimination in the exchange of α-tocopherol stereoisomers between plasma and red blood cells" 22 : 469-475, 1987

31 Lee, J. Y., "Antioxidant and anticancer activities of organic extracts from Platycodon grsndiflorum a. De Candolle roots" 93 : 409-415, 2004

32 Erkan, N., "Antioxidant activities of rosemary (Rosmarinus officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol" 110 : 76-82, 2008

33 Del Campo, J., "Antimicrobial effect of rosemary extracts" 63 (63): 1359-1368, 2000

34 Djenane, D., "Ability of a- tocopherol, taurine, and rosemary, in combination with vitamin C, to increase oxidative stability of beef steaks packaged in modified atmosphere" 76 : 407-415, 2002

35 Witte, V. C., "A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage" 35 : 352-358, 1970