<i>Staphylococcus aureus</i> PerR Is a Hypersensitive Hydrogen Peroxide Sensor using Iron-mediated Histidine Oxidation

다국어 초록 (Multilingual Abstract)

<P>In many Gram-positive bacteria PerR is a major peroxide sensor whose repressor activity is dependent on a bound metal cofactor. The prototype for PerR sensors, the Bacillus subtilis PerRBS protein, represses target genes when bound to either Mn2+ or Fe2+ as corepressor, but only the Fe2+-bound form responds to H2O2. The orthologous protein in the human pathogen Staphylococcus aureus, PerR(SA), plays important roles in H2O2 resistance and virulence. However, PerR(SA) is reported to only respond to Mn2+ as corepressor, which suggests that it might rely on a distinct, iron-independent mechanism for H2O2 sensing. Here we demonstrate that PerR(SA) uses either Fe2+ or Mn2+ as corepressor, and that, like PerRBS, the Fe2+-bound form of PerR(SA) senses physiological levels of H2O2 by iron-mediated histidine oxidation. Moreover, we show that PerR(SA) is poised to sense very low levels of endogenous H2O2, which normally cannot be sensed by B. subtilis PerRBS. This hypersensitivity of PerR(SA) accounts for the apparent lack of Fe2+-dependent repressor activity and consequent Mn2+-specific repressor activity under aerobic conditions. We also provide evidence that the activity of PerR(SA) is directly correlated with virulence, whereas it is inversely correlated with H2O2 resistance, suggesting that PerR(SA) may be an attractive target for the control of S. aureus pathogenesis.</P>