자연과학편 : 저항성 운동과 고단백식이가 중년 흰쥐 골격근의 TLR-4 단백질 활성에 의한 내재면역기능에 미치는 영향 = Effects of resistance exercise and high-protein diet treatment on innate immune function by TLR-4 protein activation on skeletal muscle of middle aged rat

국문 초록 (Abstract)

본 연구의 목적은 저항성 운동과 고단백식이가 흰쥐 골격근의 내재면역 반응과정에서 TLR-4 단백질 활성 및 염증성 사이토카인 발현에 미치는 영향을 분석하는데 있다. 연구대상은 50주령 Wistar계 중년 수컷 흰쥐 50마리를 4주간 고지방식이로 비만을 유도한 후 5집단(High fat diet+Control, High fat diet+High fat diet, High fat diet+High protein diet, High fat diet+High fat diet+Resistance exercise, High fat diet+High protein diet+Resistance exercise)으로 구분하였다. 그 후 8주간 그룹에 따라 고지방식이, 고단백식이 및 저항성 운동(사다리 운동, 체중의 70~100% 부가, 8회/세트, 8세트, 3일/주) 처치를 병행하였다. 8주간의 식이 처치 및 저항성 운동 후 골격근을 적출하여 분석하였다. 연구결과 8주간의 고지방식이 그룹에서 저항성 운동 후 인슐린 저항성, TLR-4 단백질 활성, IL-1β 및 IL-6의 발현이 감소하였으며, 고단백질 식이 그룹에서는 TLR-4 및 IL-6에서 저항성 운동의 효과가 나타났다. 그러므로 저항성 운동은 골격근 기능을 강화시켜 내재면역기능을 높이는 것으로 생각되지만 세포표면에서의 내재면역의 신호전달기전에 대한 추후 연구가 필요할 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

The purpose of this study was to investigate the effects of resistance exercise and high-protein diet treatment on innate immune function by TLR-4 protein activation and pro-inflammation cytokines on skeletal muscle of middle aged rat. 50-week middle aged male Wistar rats were given a 4-week high-fat diet to induce obesity before randomly assigning them into 5 groups (High fat diet+Control, High fat diet+High fat diet, High fat diet+High protein diet, High fat diet+High fat diet+Resistance exercise, High fat diet+High protein diet+Resistance exercise). Combinations of high fat diet, high protein diet, and resistance exercise (ladder climbing) treatments were administered. The groups with added resistance exercise were trained for 8 weeks with workout intensity, duration, and frequency of 70~100% body weight, 8rep/set, 8set, 3day/week respectively. After 8 weeks of diet treatment and resistance exercise, rats were sacrificed and skeletal muscle were dissected for analysis. Results showed insulin resistance, TLR-4 protein activation, IL-1β and IL-6 expressions significantly decreased after resistance exercise in the HF group. On the other hand, TLR-4 and IL-6 expressions of rats in the HP group also decreased following resistance exercise. Therefore, resistance exercise is believed to improve the innate immune system particularly in enhancing skeletal muscle function but further studies are needed to understand the cell surface’s innate immune pathway.

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