Sphingopyxis granuli sp. nov., a b-Glucosidase-Producing Bacterium in the Family Sphingomonadaceae in a-4 Subclass of the Proteobacteria

다국어 초록 (Multilingual Abstract)

Strain Kw07T, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from granules in an Up-flow Anaerobic Sludge Blanket (UASB) bioreactor used in the treatment of brewery wastewater.
16S rRNA gene sequence analysis revealed that strain Kw07T belongs to the α-4 subclass of the Proteobacteria, and the highest degree of sequence similarity was determined to be to Sphingopyxis macrogoltabida IFO 15033T (97.8%). Chemotaxonomic data revealed that strain Kw07T possesses a quinone system with the predominant compound Q-10, the predominant fatty acid C18:1 ω7c, and sphingolipids, all of which corroborated our assignment of the strain to the Sphingopyxis genus.
The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Kw07T represents a distinct species. Based on these data, Kw07T (= KCTC 12209T = NBRC 100800T) should be classified as the type strain for a novel Sphingopyxis species, for which the name Sphingopyxis granuli sp. nov. has been proposed.

참고문헌 (Reference)

1 Buck, "method for determination ofGram reactions of marine bacteria" (nstaining) : 992-993, 1982

2 Li, "isolated from theRussian space laboratory Mir" 54 : 819-825, 2004

3 Yoon, "isolated from sea water of the Yellow Sea in Korea" 55 : 369-373, 2005

4 Rivas, "from the phyllosphere ofAcacia caven in Argentina" 54 : 2147-2150, 2004

5 Thompson, "flexible strategiesfor multiple sequence alignment aided by quality analysistools" 24 : 4876-4882, 1997

6 Fujii, "andoestradiol-degrading bacterium isolated from activated sludgeof a sewage treatment plant in Tokyo" 53 : 47-52, 2003

7 Godoy, "achlorophenol-degrading bacterium that accumulates polyhydroxyalkanoate and transfer of Sphingomonas alaskensis toSphingopyxis alaskensis comb" 53 : 473-477, 2003

8 Hall, "a user-friendly biological sequence alignmenteditor and analysis program for Windows 95/98/NT" 95-98, 1999

9 Goebel, "a placefor DNA-DNA reassociation and 16S rRNA sequence analysisin the present species definition in bacteriology" 846-849, 1994

10 Bio, "a newmethod for reconstructing phylogenetic trees" e : 406-425, 1987

11 Sohn, "a highmolecular-mass polycyclic aromatic hydrocarbon-degradingbacterium isolated from estuarine sediment" 54 : 1483-1487, 2004

12 Schut, "a dominantbacterium from a marine oligotrophic environment" 51 : 73-79, 2001

13 Lettinga, "Use of anaerobic digestionfor wastewater treatment" 110-112, 1980

14 Kimura, "The Neutral Theory of Molecular Evolution" Cambridge University Press tionary genetics analysis software : 1244-1245, 2001

15 Takeuchi, "Taxonomicstudy of polyethylene glycol-utilizing bacteria emendeddescription of the genus Sphingomonas and new descriptions ofSphingomonas macrogoltabidus sp" (v v v) : 227-238, 1993

16 I, "Proposals of Sphingomonas paucimobilisgen andtwo genospecies of the genus Sphingomonas" ya sphingomonas ya immu (ya sphingomonas ya immu): 99-119, 1990

17 Takeuchi, "Proposal of thegenus Sphingomonas sensu stricto and three new genera" 51 : 1405-1417, 2001

18 U, "Precisemeasurement of the G+C content of deoxyribonucleic acid byhigh-performance liquid chromatography" 159-167, 1989

19 MacLeod, "Layeredstructure of biological aggregates produced in an upflow sludgebed and filter reactor" 1598-1607, 1990

20 Shin, "Isoprenoid quinine profiles of the Leclercia adecarboxylataKCTC 1036T" 6 : 68-69, 1996

21 Sasser, "Identification of bacteria by gas chromatographyof cellular fatty acids" MIDI Inc 1990

22 Hashimoto, "Fluorometricdeoxyribonucleic acid-deoxyribonucleic acid hybridization inmicrodilution wells as an alternative to membrane filter hybridizationin which radioisotopes are used to determine geneticrelatedness among bacterial strains" 224-229, 1989

23 Fujiwara, "Emendation of the genus SphingomonasYabuuuchi et al 1990 and junior objective synonymyof the species of three genera in conjunction with Blastomonasursincola" 52 : 1485-1496, 2002

24 Sherman, "Distribution of isoprenoidquinone structural types in bacteria and their taxonomic implications" Pearson Education, Inc., 2002

25 Lett, "Determination of DNA basecomposition by reversed phase high-performance liquid chromatography" 125-128, 1984

26 an approachusing the bootstrap, "Confidence limit on phylogenies" tion 39 : 783-791, 1985

27 Chang, "Characteristicsof granular methanogenic sludges grown on lactate ina UASB reactor" 465-472, 1991

28 Schmidt, "Acetate and hydrogen metabolismin intact and disintegrated granules from an acetate-fed" 49 : 229-246, 1996

29 Barns, "16S ribosomal DNA amplification for phylogenetic study" 697-173 703, 1991