고온, 건조 스트레스 조건에서 멜라토닌 경엽처리에 의한 배추의 생장 및 항산화효소 활성 증대

국문 초록 (Abstract)

멜라토닌은 작물의 생장 및 분화 그리고 비생물적 환경 스트레스 조건에서 다양한 생리적 기능을 조절하는 물질로 알려져 있다. 본 연구에서는 고온과 건조 스트레스 조건에서 배추에 멜라토닌을 세가지 수준의 농도(5, 10 및 20mg·L<SUP>-1</SUP> )로 엽면 살포하고, 스트레스 경감에 효과적인 농도를 찾고자 하였다. 고온 및 건조 스트레스 조건에서, 10mg·L<SUP>-1</SUP> 농도의 멜라토닌 처리구의 생체중과 엽면적이 대조구에 비해 각각 22.7%와 13.9%씩 증가함을 확인하였다. 광합성 특성 측정결과, 10mg·L<SUP>-1</SUP> 처리구에서 광합성 속도와 증산율이 대조구에 비해 유의한 차이를 보이며 증가하였고, 과산화수소(H₂O₂) 함량도 가장 낮게 측정되었다. 반면, 여러 농도의 멜라토닌 처리에 의한 MDA함량변화는 관찰되지 않았다. 특히, 10mg·L<SUP>-1</SUP> 처리구에서 항산화효소인 ascorbate peroxidase(APX)와 superoxide dismutase(SOD)의 활성이 대조구에 비해 증가하지만, ascorbic acid(AsA) 및 proline의 함량은 변화를 보이지 않았다. 이러한 결과들을 종합해 볼 때, 10mg·L<SUP>-1</SUP> 농도의 멜라토닌 경엽처리는 배추의 항산화효소 활성을 증가시켜 고온 및 건조 스트레스 조건에서 배추 생장에 긍정적 효과를 유발함을 확인하였다.

다국어 초록 (Multilingual Abstract)

Melatonin, an indoleamine molecule, regulates a wide range of physiological functions during the growth, morphogenesis, and response of plants to abiotic stresses. We examined the effect of exogenous melatonin application (5, 10, and 20 mg·L<SUP>-1</SUP>) on the growth and antioxidant enzyme activity of Kimchi cabbage under high temperature (air temperature 32/26°C) and drought stress (soil moisture 20-24%) conditions. Application of 10 mg·L<SUP>-1</SUP> melatonin alleviated stress-induced growth inhibition and increased the shoot fresh weight and the leaf surface area of seedlings by 22.7% and 13.9%, respectively, compared to the control under the stress condition. Indeed, foliar application of melatonin at 10 mg·L<SUP>-1</SUP> caused an increase of net photosynthetic rate and transpiration rate under the stress condition. The lowest level of H₂O₂ content was also observed at 10 mg·L<SUP>-1</SUP> concentration of melatonin treatment, while no significant alterations in lipid peroxidation or concentrations of malondialdehyde were detected by treatment with different concentrations of melatonin. In addition, significantly elevated activity levels of ascorbate peroxidase (APX) and superoxide dismutase (SOD) were measured at 10 mg·L<SUP>-1</SUP> melatonin treatment without significant changes in ascorbic acid (AsA) and proline contents under the stress condition. These results suggest that foliar application of 10 mg·L<SUP>-1</SUP> melatonin ameliorates physiological damages caused by the combination of water deficit and heat stress and enhances the activity of the antioxidant enzymes such as APX and SOD, thereby improving tolerance against the water deficit and heat stress in Kimchi cabbage.

목차 (Table of Contents)

• Abstract
• 서언
• 재료 및 방법
• 결과 및 고찰
• 초록
• Literature Cited

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