Design optimization of synthesis of composite calcium hydroxide loaded with ZnO and Ag nanoparticles as an antibacterial Agent

다국어 초록 (Multilingual Abstract)

Antibiotic resistance has become the major global health hazard that threatens people of all ages. The most important wayto reduce infectious diseases in the treatment of the root canal is the elimination of infections caused by the accumulationof bacteria. The objective of this investigation was to enhance the production process of a newly developed nanocompositecomposed of calcium hydroxide, zinc oxide, and silver. The intention behind this optimization was to achieve the utmosteffectiveness in combating Enterococcus faecalis infection by exhibiting strong antibacterial properties. To fabricatenanocomposite with the best performance, the effects of three factors of calcium hydroxide (Ca(OH)2), zinc oxide (ZnO) andsilver (Ag) nanoparticles (NPs), were investigated in three levels and nine different experiments designed using the Taguchimethod. The successful formation of a nanocomposite with desired structural properties was confirmed through variousanalytical techniques including FTIR, UV-vis, XRD, SEM, EDX, X-Ray Map, TEM, and TGA. The findings indicated thatthe produced nanocomposite when subjected to the experimental circumstances of calcium hydroxide at a concentration of50 mg/ml, ZnO at a concentration of 2 mg/ml, and Ag at a concentration of 2 mg/ml, exhibited the most potent antibacterialefficacy (0.26 CFU/ml) against the biofilm of Enterococcus faecalis. Due to the favorable antibacterial properties of thisnanocomposite, its use as an in-channel drug can lead to more successful root canal treatments.