단일 생약재 및 생약복합제 에탄올추출물의 항산화 및 항염증 활성

국문 초록 (Abstract)

본 연구에서는 문헌을 통하여 항산화 혹은 항염증 활성을 가지는 6종의 생약재와 이들 생약재를 혼합하여 제조한 생약복합제의 항산화 및 항염증 활성을 측정하였다.
총 폴리페놀 함량은 어성초, 홍화, MIX-2 에탄올추출물이 각각 58.98, 60.79, 57.74 mg/g의 함량으로 다른 시료들 보다 높게 측정되었다. 또한 플라보노이드 함량도 어성초(HC), 홍화(CT), MIX-2 에탄올추출물이 각각 36.86, 19.42, 14.74 mg/g으로 높게 측정되었다. 단일 생약재와 생약복합제 에탄올추출물의 라디칼 소거능을 측정한 결과 DPPH 라디칼의 RC<SUB>50</SUB> 값은 어성초, 홍화, MIX-2 에탄올추출물에서 각각 69.64, 50.70, 63.49 μg/mL로 추출물 가운데 우수한 DPPH 라디칼 소거능을 보였고, ABTS 라디칼의 RC<SUB>50</SUB> 값도 어성초, 홍화, MIX-2가 각각 24.06, 18.91, 41.61 μg/mL로 DPPH 라디칼 소거능과 유사한 경향을 보였다. 시료들의 항염증 효능을 측정하기 위해 5-LO 및 COX-2 효소 저해활성을 측정한 결과 단일 생약재 에탄올추출물보다 생약복합제 에탄올추출물이 5-LO와 COX-2 효소를 동시에 저해하는 효과가 증가되는 것으로 나타났다. 염증유발 효소 저해활성이 우수했던 생약복합제 에탄올추출물들의 NO 소거활성을 비교한 결과, MIX-2 에탄올추출물이 50 μg/mL의 농도에서부터 유의적으로 NO의 생성을 저해하는 것을 확인하였다. 따라서 단일 생약재 보다는 생약복합제가 서로 혼합되면서 시너지 효과가 나타나는 것으로 생각되며 앞으로 명확한 기전에 대한 연구들이 지속된다면 기능성 식품 소재로서의 이용이 가능할 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

In this study, antioxidant and anti-inflammatory effects of ethanol extracts from medicinal herb mixtures were investigated in vitro. Contents of total polyphenols in ethanol extracts from Houttuynia cordata Thunb. (HC), Carthamus tinctorius L. (CT), and MIX-2 (Houttuynia cordata Thunb., Acanthopanax gracilistylus Smith, Carthamus tinctorius L., Kalopanax pictus) were 58.98, 60.79 and 57.74 mg/g respectively. Radical scavenging activities of the ethanol extracts were examined using α,α-diphenyl-β-picrylhydrazyl radicals and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay. Anti-inflammatory effects of ethanol extracts from medicinal herbs composites were examined using 5-lipoxygenase (5-LO), cyclooxygenase-2 (COX-2) and nitric oxide (NO) inhibition assays. The ethanol extracts from medicinal herb mixtures showed higher 5-LO and COX-2 inhibition activities than those from single medicinal herbs. The ethanol extracts from medicinal herb mixtures also showed strong anti-inflammatory activity through inhibition of NO production.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 감사의 글
• 문헌

참고문헌 (Reference)

1 노경호, "천연물에 포함된 미백성분의 추출 및 미백효과" 한국공업화학회 16 (16): 348-353, 2005

2 안선일, "일부 페놀성 화합물의 항산화효과 및 아질산염 소거능" 한국식품조리과학회 23 (23): 19-24, 2007

3 안영희, "울릉도의 섬자리공 분포와 자생지의 생태적 특성" 한국생약학회 38 (38): 1-9, 2007

4 송종호, "어성초(Houttuynia cordata) 뿌리에서 추출한 순차분획물의 항균활성" 한국식품영양과학회 32 (32): 1053-1058, 2003

5 이성규, "아보카도 추출물의 Apoptosis 유도와 항산화 활성" 한국식품영양과학회 37 (37): 269-275, 2008

6 유미희, "생대추(풋대추) 과육 및 씨 추출물의 항산화 효과 및 항산화 성분" 한국식품과학회 38 (38): 128-134, 2006

7 이명희, "기능성 천연물 복합제의 이화학적 특성 확인" 한국식품영양과학회 36 (36): 1571-1577, 2007

8 이용숙, "金銀花와 黃芩이 配伍된 處方劑의 抗炎症 效果 硏究" 대한한의학방제학회 18 (18): 121-132, 2010

9 Choe SY, "Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and butylated hydroxy anisol (BHA)" 14 : 283-288, 1982

10 Barene AL, "Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytolune" 52 : 59-63, 1975

11 Cho EJ, "The inhibitory effects of 12 medicinal plants and their component on lipid perocidation" 31 : 907-917, 2003

12 Sadaki O, "The development of functional and materials" 13 : 44-50, 1996

13 Kang YH, "Studies on the physiological functionality of pine needle and mugwort extracts" 27 : 978-984, 1995

14 Kawashima S, "Serotonin derivative, N-(ρ-coumaroyl) serotonin, inhibits the production of TNF-α, IL-1α. IL-1β, and IL-6 by endotoxin-stiumlated human blood monocytes" 18 : 423-428, 1998

15 Yang MS, "Screening of domestic plants with antibacterial activity" 38 : 548-589, 1995

16 Kim KM, "Screening of anti-allergic agents using lipoxygenase assay and degranulation marker" 5 : 45-48, 1996

17 Cho SY, "Screening for antioxidant activity of edible plants" 30 : 133-137, 2001

18 Kim KD, "Research of oriental medicine plant on antioxidation and ultraviolet rays absorption" 10 : 145-153, 2004

19 Halliwell B, "Reactive oxygen species and the central nervous system" 59 : 1609-1623, 1992

20 Fubini B, "Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis" 34 : 1507-1516, 2003

21 Funk CD, "Prostaglandins and leukotrienes; advances in eicosanoid biology" 294 : 1871-1875, 2001

22 Pratt DE, "Phenolic Compounds in Food and Their Effects on Health (II)" antioxidants and cancer prevention 54-71, 1992

23 Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents" 12 : 239-249, 1912

24 Gross SS, "Nitric oxide: Pathophysiological mechanisms" 57 : 737-769, 1995

25 Cadenas E, "Mitochondrial free radical generation, oxidative stress, and aging" 29 : 222-230, 2000

26 Kim DH, "Metabolism of kalopanax B and H by human intestinal bacteria and antidiabetic activity of their metabolites" 21 : 360-365, 1998

27 Stuehr DJ, "Mammalian nitric oxide synthases" 1411 : 271-230, 1999

28 Choi JW, "Kalopanaxsaponin A from kalopanax pictus, a potent antioxidant in the rheumatoidal rat treated with Freund's complete adjuvant reagent" 79 : 113-118, 2002

29 Li Y, "Improvement in the turnover rate of the stratum comeum in false aged model rats by the administration of geniposide acid in Eucommia ulmoides oliver leaf" 22 : 582-585, 1999

30 Giles GI, "Hypothesis: The role of reactive sulfur species in oxidative stress" 31 : 1279-1283, 2001

31 Singh RK, "Further study of anti-inflammatory effects of Abies pindrow" 11 : 535-537, 1997

32 Fang YZ, "Free radicals, antioxidants, and nutrition" 18 : 872-879, 2002

33 Charmichael J, "Evaluation of a tetrazolium based semiautomated colorimetric assay, assessment of chemosensitivity testing" 47 : 936-941, 1987

34 Kaliora AC, "Dietary antioxidants in preventing atherogenesis" 187 : 1-17, 2006

35 Bae SJ, "Cytotoxicity of phenolic compounds isolated from seeds of safflower (Carthamus tinctorius L.) on cancer cell lines" 11 : 140-146, 2002

36 Nieva, Moreno MI, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina" 71 : 109-114, 2000

37 Bansky D, "Chinese Herbal Medicine Formulas and Strategies" Eastland Press 7-8, 1990

38 Zhang HL, "Antioxidative dompounds isolated from safflower (Carthamus tinctorious L.) oil cake" 45 : 1910-1914, 1997

39 Moini G, "Antioxidant and prooxidant activities of α-lipoic acid and dihydrolipoic acid" 182 : 84-90, 2002

40 Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay" 26 : 1231-1237, 1999

41 Lee MW, "Antifungal acticity of modified hederagenin glycosides from the leaves of Kalopanax pictum var. chinense" 24 : 718-719, 2001

42 Choi L, "Anti-lipid peroxidative principles from the stem bark of Kalopanax pictus Nakai" 24 : 536-540, 2001

43 Lee EB, "Anti-inflammatory activity of methanol extract of Kalopanax pictus bark and its fractions" 77 : 197-201, 2001

44 Green LC, "Analysis of nitrate, and nitrate in biological fluids" 126 : 131-138, 1982

45 Chen YY, "A study of antioxidative and antimutagenic effects of Houttuynia cordata thunb using an oxidized frying oil-fed model" 49 : 327-333, 2003