딸기 온실에서 점박이응애의 축차표본조사법 개발 = Development of Sequential Sampling Plans for Tetranychus urticae in Strawberry Greenhouses

국문 초록 (Abstract)

두 개의 딸기 온실 (농약살포포장, 천적방사포장)에서 점 박이응애 (Tetranychus urticae Koch)의 고정 정확도 수준에 서 표본조사법 (Fixed-precision sampling plan)을 개발하였 다. 표본추출은 조사구역당 3개엽으로 이루어진 복엽 1줄기 를 기준으로 하여 3줄기를 채취하였다. 각 복엽은 Relative net precision (RNP) 값을 비교하기 위해 3개의 서로 다른 단 위 (1엽, 2엽, 3엽)로 나뉘어졌다. RNP 값 결과 1엽 단위가 다 른 단위들보다 정확도와 효율적인 면에서 우수했다. 공간분 포 분석은 Taylor’s power law (TPL)를 이용하였으며, 각 딸 기 온실별로 계산된 TPL 계수의 동질성검정에는 공분산분 석 (ANCOVA)을 이용하였으며, 분석결과 차이를 보이지 않 았다. 표본추출 정시선을 구하기 위한 TPL 계수는 농약살포 포장과 천적방사포장의 딸기 1엽 단위에서 점박이응애 밀 도 자료를 합한 뒤 재계산하여 사용하였다. 그리고 점박이 응애 발생밀도수준을 3마리와 10마리로 설정하여 방제의사 를 결정하였다. 분석에 사용하지 않은 독립된 자료를 이용 하여 개발된 표본추출법의 유효성을 Resampling Validation for sampling plan (RVSP)으로 확인한 결과 적합한 정확도를 보였다.

다국어 초록 (Multilingual Abstract)

A fixed-precision-level sampling plan was developed to establish control of the two-spotted spider mite, Tetranychus urticae, in two strawberry greenhouses (conventional plot, natural enemy plot). T. urticae was sampled by taking a three-leaflet leaf (1 stalk) from each plant (3 three-leaflet leaves) from each sampling position. Each leaflet was divided into three different units (1-leaflet, 2-leaflet, and 3-leaflet units) to compare relative net precision (RNP) values for selection of the appropriate sampling unit. The relative net precision values indicated that a 1-leaflet unit was more precise and cost-efficient than other units. The spatial distribution analysis was performed using Taylor’s power law (TPL). Homogeneity of the TPL parameters in each greenhouse was evaluated by using the analysis of covariance (ANCOVA). A fixed-precision-level sequential sampling plan was developed using the parameters of TPL generated from the combined data of the conventional plot and natural enemy plot in a 1-leaflet sampling unit. Sequential classification sampling plans were also developed using the action threshold of 3 and 10 mites for pooled data. Using the results obtained in the independent data, simulated validation of the developed sampling plan by Resampling validation for sampling plan (RVSP) indicated a reasonable level of precision.

참고문헌 (Reference)

1 김태일, "촉성 재배용 딸기 신품종 '매향' 육성" 한국원예학회 22 (22): 434-437, 2004

2 박석희, "참외에서 담배가루이의 경제적 피해수준 설정" 한국농약과학회 18 (18): 196-201, 2014

3 박홍현, "시설재배 가지, 피망에서 꽃노랑총채벌레 피해해석과 방제수준" 한국응용곤충학회 48 (48): 229-236, 2009

4 최용석, "딸기 시설재배지에서 점박이응애의 발생과 분포" 농업과학연구소 41 (41): 315-319, 2014

5 Naranjo SE, "Spatial distribution of adult Bemisia tabaci (Homoptera: Aleyrodidae) in cotton and development and validation of fixed-precision sampling plans for estimating population density" 24 : 261-270, 1995

6 Wise IL, "Spatial distribution and sequential sampling methods for the potato aphid, Macrosiphum euphorbiae (Thomas) (Homoptera: Aphididae), in oilseed flax" 127 : 967-976, 1995

7 Park JJ, "Sampling plans for estimation and classifying population density of two spotted mite (Acari: Tetranychidae) on the greenhouse rose cultured with arching method" 29 : 127-134, 1999

8 Binns MR, "Sampling insect population for the purpose of IPM decision making" 37 : 427-453, 1992

9 Ruesink WG, "Sampling Methods in Soybean Entomology" Springer 61-78, 1980

10 SAS Institute, "SAS user’s guide"

11 Green RH, "On fixed precision level sequential sampling" 12 : 249-251, 1970

12 Laing JE, "Life history and life table of Tetranychus urticae Koch" Acarologia 32-42, 1969

13 Croft BA, "Inheritance of early-stage resistance to formetanate and cyhexatin in Tetranychus urticae Koch (Acarina: Tetranychidae)" 77 : 575-578, 1984

14 Hutchison WD, "Implications of the stochastic nature of Kuno’s and Green’s fixed-precision stop lines: sampling plans for the pea aphid (Homoptera: Aphididae) in alfalfa as an example" 81 : 749-758, 1988

15 Pedigo LP, "Handbook of Sampling Methods for Arthropods in Agriculture" CRC press 1-11, 1994

16 Naranjo SE, "Handbook of Pest Management in Agriculture, Vol. 2" CRC Press 67-132, 1997

17 Hodgson EW, "Enumerative and Binomial Sequential Sampling Plans for Soybean Aphid (Homoptera: Aphididae) in Soybean" 97 : 2127-2136, 2004

18 Pedigo LP, "Entomology and Pest Management" Macmillian Publish 1989

19 Southwood TRE, "Ecological methods" Chapman and Hall 1978

20 Korea Crop Protection Association, "Crop protection Guidelines"

21 Sokal RR, "Biometry" W. H. Freeman and Company 1981

22 Taylor LR, "Aggregation, variance and the mean" 189 : 732-735, 1961

23 Kuno, E., "A new method of sequential sampling to obtain the population estimates with a fixed level of precision" 11 : 127-136, 1969

24 Iwao, S., "A new method of sequential sampling to classify populations relative to a critical density" 16 : 281-288, 1975